Ley R D
Pathophysiology Division, Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87108, USA.
Cancer Res. 1997 Sep 1;57(17):3682-4.
Two groups of 30 dorsally shaved opossums (Monodelphis domestica) were exposed three times per week for 81 weeks to 250 J/m2 of UV radiation from FS40 sunlamps (approximately 150 J/m2 of UV radiation B; UV-B), or to 2.5 x 10(4) J/m2 of UV radiation A (UV-A) from filtered F40BLB fluorescent lamps (black lights). Animals were monitored for the appearance of nonmelanoma skin tumors (NMSTs) and melanocytic hyperplasia (MH). After 81 weeks of exposures, the prevalence of NMSTs was 71% and 4% for animals exposed to UV-B and UV-A, respectively. The difference between the treatment groups was statistically significant (P < 0.001). However, the prevalence of MH in the treatment groups, 31% for UV-B-exposed animals and 22% for UV-A-exposed animals, was not significantly different (P > 0.05). Thus, a dose of UV-A that was relatively ineffective in producing NMSTs, compared to UV-B, was as effective as UV-B in the induction of MH. If, as shown previously, MH is the precursor lesion for melanoma in this model, these results suggest that the action spectra for the induction of melanoma and NMSTs in the opossum are different.
两组各30只背部剃毛的负鼠（家短尾负鼠），每周接受3次照射，持续81周，一组接受来自FS40太阳灯的250 J/m²紫外线辐射（约150 J/m²的紫外线B；UV-B），另一组接受来自过滤后的F40BLB荧光灯（黑光灯）的2.5×10⁴ J/m²紫外线A（UV-A）。监测动物非黑素瘤皮肤肿瘤（NMSTs）和黑素细胞增生（MH）的出现情况。照射81周后，接受UV-B和UV-A照射的动物中，NMSTs的发生率分别为71%和4%。治疗组之间的差异具有统计学意义（P < 0.001）。然而，治疗组中MH的发生率，UV-B照射的动物为31%，UV-A照射的动物为22%，差异无统计学意义（P > 0.05）。因此，与UV-B相比，在产生NMSTs方面相对无效的UV-A剂量，在诱导MH方面与UV-B一样有效。如果如先前所示，在该模型中MH是黑色素瘤的前体病变，这些结果表明负鼠中黑色素瘤和NMSTs诱导的作用光谱是不同的。
