Mendling W, Pinto De Andrade M, Gutschmidt J, Gantenberg R, Presber W, Schönian G
Klinik für Frauenheilkunde und Geburtshilfe, Perinatalzentrum, Klinikum Frankfurt/Oder, Germany.
Mycoses. 2000;43(11-12):387-92.
Sexual partners often harbour identical yeast strains in the vagina, in the orointestinal tract and in semen in cases of recurrent vulvovaginal candidoses. Specimen were collected from vagina, oral cavity and faeces of the patients, and from semen, oral cavity and faeces of their male partners. Mycological cultures were grown on Sabouraud glucose-agar and, if positive, specified by Candida-ID-Agar (BioMérieux), by formation of chlamydospores on rice agar, and by biochemotyping with the System Walkaway (Dade) or the API-32C system (BioMérieux). A polymerase chain reaction finger-printing technique with the T3B oligonucleotide as single primer was used for strain typing. Candida albicans was isolated from the vagina of 18 out of 21 patients, the vagina of one patient harboured a strain of Candida glabrata. The cultures obtained from vagina, oral cavity and faeces were genetically identical in 12 patients. From the partners of 15 patients C. albicans was cultured in at least one of the clinical samples. Identical strains were observed for eight of 15 couples, whereas four of these identical strains were cultured from semen. Further prospective investigations will prove whether a consequent treatment of both partners will eradicate identical yeast strains and will be able to improve the results of treatment in such women.
在复发性外阴阴道念珠菌病病例中，性伴侣的阴道、口肠道和精液中常携带相同的酵母菌菌株。从患者的阴道、口腔和粪便以及其男性伴侣的精液、口腔和粪便中采集样本。真菌培养物在沙保弱葡萄糖琼脂上生长，若呈阳性，则通过念珠菌鉴定琼脂（生物梅里埃公司）、在大米琼脂上形成厚垣孢子以及使用系统沃克威（达德公司）或API-32C系统（生物梅里埃公司）进行生化分型来鉴定。使用以T3B寡核苷酸作为单一引物的聚合酶链反应指纹技术进行菌株分型。21例患者中有18例从阴道分离出白色念珠菌，1例患者的阴道中携带光滑念珠菌菌株。12例患者阴道、口腔和粪便的培养物基因相同。在15例患者的伴侣中，至少在一份临床样本中培养出白色念珠菌。15对伴侣中有8对观察到相同菌株，其中4对相同菌株是从精液中培养出来的。进一步的前瞻性研究将证明对双方伴侣进行连续治疗是否能根除相同的酵母菌菌株，并能否改善此类女性的治疗效果。
