Southern Peter, Horbul Julie, Maher Diane, Davis Dana A
Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, United States of America.
PLoS One. 2008 Apr 30;3(4):e2067. doi: 10.1371/journal.pone.0002067.
Candida albicans is a low level commensal organism in normal human populations with the continuous potential to expand and cause a spectrum of clinical conditions.
METHODOLOGY/PRINCIPAL FINDINGS: Using ex vivo human organ cultures and populations of primary human cells, we have developed several related experimental systems to examine early-stage interactions between C. albicans and mucosal surfaces. Experiments have been conducted both with exogenously added C. albicans and with overtly normal human mucosal surfaces supporting pre-existing infections with natural isolates of Candida. Under different culture conditions, we have demonstrated the formation of C. albicans colonies on human target cells and filament formation, equivalent to tissue invasion.
CONCLUSIONS/SIGNIFICANCE: These organ culture systems provide a valuable new resource to examine the molecular and cellular basis for Candida colonization of human mucosal surfaces.
白色念珠菌在正常人群中是一种低水平的共生生物体，持续具有扩张并引发一系列临床病症的可能性。
方法/主要发现：利用体外人体器官培养物和原代人体细胞群体，我们开发了多个相关实验系统，以研究白色念珠菌与黏膜表面之间的早期相互作用。我们既对外源添加的白色念珠菌进行了实验，也对支持念珠菌自然分离株既往感染的明显正常人体黏膜表面进行了实验。在不同培养条件下，我们证明了白色念珠菌在人体靶细胞上形成菌落以及形成菌丝，这等同于组织侵袭。
结论/意义：这些器官培养系统为研究白色念珠菌在人体黏膜表面定植的分子和细胞基础提供了宝贵的新资源。
