Morgenstern S, Koren R, Fraser G, Okon E, Niv Y
Department of Pathology, Rabin Medical Center, Tel-Aviv University, Israel.
J Clin Gastroenterol. 2001 Mar;32(3):218-21. doi: 10.1097/00004836-200103000-00007.
Twelve different genes for mucin have been described. MUC5AC and MUC6 encode the secreted apomucins of the stomach. A gradient from the surface epithelium (foveola) to the glands is typical for MUC5AC synthesis, whereas a gradient in the opposite direction was found for MUC6. Our goal was to determine the distribution of MUC5AC and MUC6 in the postoperative stomach, with relation to the H. pylori status. Gastric corpus biopsy specimens from patients who underwent partial gastrectomy were examined by immunohistochemistry for mucin gene (MUC5AC and MUC6) apoproteins. We used polyclonal antibodies for amino acid tandem repeats of both proteins. A scoring system (0-3) was used to assess staining intensity at four sites: the lumen, the foveola, the mucous neck cells, and the glands. Helicobacter pylori status was determined by histology and rapid urease test and was considered positive or negative when both tests were positive or negative, respectively. We studied 19 H. pylori-positive and 32 H. pylori-negative patients. No significant change in MUC5AC or MUC6 synthesis and secretion was demonstrated between H. pylori-positive or -negative patients. A gradient similar to that shown for the intact stomach (from the surface epithelium to the glands) for MUC5AC protein and an increase of MUC6 protein presentation from the mucous neck cell to the glands were demonstrated. The pattern of MUC5AC protein synthesis was not different between H. pylori-positive and -negative patients in the postoperative stomach. MUC6 expression was higher in the foveola in H. pylori-positive patients, whereas there was no difference in the other cell layers.
已经描述了12种不同的粘蛋白基因。MUC5AC和MUC6编码胃分泌的脱辅基粘蛋白。MUC5AC合成的典型特征是从表面上皮（小凹）到腺体呈梯度变化，而MUC6则呈现相反方向的梯度变化。我们的目标是确定MUC5AC和MUC6在术后胃中的分布，并与幽门螺杆菌状态相关联。对接受部分胃切除术患者的胃体活检标本进行免疫组织化学检查，以检测粘蛋白基因（MUC5AC和MUC6）的载脂蛋白。我们使用了针对这两种蛋白质氨基酸串联重复序列的多克隆抗体。采用评分系统（0-3）评估四个部位的染色强度：管腔、小凹、粘液颈细胞和腺体。幽门螺杆菌状态通过组织学和快速尿素酶试验确定，当两项试验均为阳性或阴性时，分别视为阳性或阴性。我们研究了19例幽门螺杆菌阳性和32例幽门螺杆菌阴性患者。幽门螺杆菌阳性或阴性患者之间未显示出MUC5AC或MUC6合成与分泌有显著变化。显示出与完整胃相似的MUC5AC蛋白梯度（从表面上皮到腺体），以及MUC6蛋白从粘液颈细胞到腺体的表达增加。术后胃中幽门螺杆菌阳性和阴性患者之间MUC5AC蛋白合成模式没有差异。幽门螺杆菌阳性患者小凹中MUC6表达较高，而在其他细胞层中没有差异。
