Watanabe K, Hamano S, Yada S, Noda K, Kishihara K, Nomoto K, Tada I
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.
Parasitol Res. 2001 Feb;87(2):149-54. doi: 10.1007/pl00008568.
The effect of interleukin-4 (IL-4) on the induction of intestinal mast cells and cytokine profiles during Strongyloides ratti infection was studied using IL-4 knockout (IL-4 KO) mice. The antigen-specific proliferative response of mesenteric lymph node cells was not impaired in IL-4 KO mice. The number of intestinal mast cells induced in IL-4 KO mice during S. ratti infection was 2- to 3-fold lower than that observed in WT mice. Intestinal mastocytosis had disappeared in IL-4 KO mice by day 21 postinfection, when significant mastocytosis continued to be observed in WT mice. In mesenteric lymphnode of IL-4 KO, IL-3 production decreased and mice IFN-gamma production significantly increased as compared with those of WT mice. The numbers of eggs excreted per gram of feces (EPG) by IL-4 KO mice were greater than those excreted by WT mice on day 6 postinfection, but no difference was observed in the subsequent period. In conclusion, intestinal mast cells are induced during S. ratti infection in the absence of IL-4, and IL-4 is not essential for protection against intestinal adult worms of S. ratti.
利用白细胞介素-4（IL-4）基因敲除（IL-4 KO）小鼠，研究了IL-4对大鼠类圆线虫感染期间肠道肥大细胞诱导及细胞因子谱的影响。IL-4 KO小鼠肠系膜淋巴结细胞的抗原特异性增殖反应未受损。在大鼠类圆线虫感染期间，IL-4 KO小鼠诱导产生的肠道肥大细胞数量比野生型（WT）小鼠低2至3倍。感染后第21天，IL-4 KO小鼠的肠道肥大细胞增多症消失，而WT小鼠中仍持续观察到明显的肥大细胞增多症。与WT小鼠相比，IL-4 KO小鼠肠系膜淋巴结中IL-3的产生减少，干扰素-γ（IFN-γ）的产生显著增加。感染后第6天，IL-4 KO小鼠每克粪便中排出的虫卵数（EPG）高于WT小鼠，但在随后的时期未观察到差异。总之，在没有IL-4的情况下，大鼠类圆线虫感染期间可诱导肠道肥大细胞，且IL-4对于抵抗大鼠类圆线虫肠道成虫并非必不可少。
