Abe T, Sugaya H, Yoshimura K
Department of Parasitology, Akita University School of Medicine, Japan.
J Helminthol. 1998 Mar;72(1):1-8. doi: 10.1017/s0022149x00000894.
T cell populations and IL-3 mRNA expression were analysed in mesenteric lymph node cells and intestinal intraepithelial lymphocytes (IEL) in Strongyloides ratti-infected mice. On days 7 and 12 post-infection, 2.6 times as many mesenteric lymph node cells were present in S. ratti-infected mice compared with uninfected mice. Although the percentages of CD3+, CD4+ and CD8+ cells decreased during infection, the absolute numbers of these cell types increased on day 7 due to an overall increase in the mesenteric lymph node cell number. The CD4/CD8 ratio in IEL was increased on day 5, whereas no significant change in the CD4/CD8 ratio was observed in the mesenteric lymph node cells. Expression of IL-3 mRNA, which is an important cytokine for the induction of murine mucosal mastocytosis and S. ratti-expulsion, was examined in mesenteric lymph nodes and IEL of uninfected and infected mice. IL-3 mRNA was detected in mesenteric lymph nodes of S. ratti-infected mice but not detected in the lymph nodes of uninfected mice. IL-3 mRNA was detected in IEL from both infected and uninfected mice with an 20-fold increase in expression in IEL of infected mice. Overall, IL-3 mRNA levels were higher in IEL than in mesenteric lymph nodes following S. ratti-infection. Expression of IL-4, IL-10, stem cell factor (SCF or c-kit ligand) and IFN-gamma mRNA was also examined in these two tissues. IL-10 mRNA was not detected in any tissue examined and IFN-gamma mRNA levels were unaltered as a result of an S. ratti-infection. Elevated expression of mRNA for SCF (5-fold) and IL-4 (20-fold) was observed in the mesenteric lymph nodes of infected mice. In contrast, SCF mRNA levels were similar in IEL of uninfected and infected animals and only a modest increase in IL-4 mRNA was observed in IEL of infected mice.
对感染大鼠类圆线虫的小鼠的肠系膜淋巴结细胞和肠上皮内淋巴细胞（IEL）中的T细胞群体及IL-3 mRNA表达进行了分析。在感染后第7天和第12天，感染大鼠类圆线虫的小鼠体内的肠系膜淋巴结细胞数量是未感染小鼠的2.6倍。虽然感染期间CD3⁺、CD4⁺和CD8⁺细胞的百分比下降，但由于肠系膜淋巴结细胞数量总体增加，这些细胞类型的绝对数量在第7天有所增加。IEL中的CD4/CD8比值在第5天升高，而肠系膜淋巴结细胞中的CD4/CD8比值未观察到显著变化。在未感染和感染小鼠的肠系膜淋巴结及IEL中检测了IL-3 mRNA的表达，IL-3是诱导小鼠黏膜肥大细胞增多症和驱除大鼠类圆线虫的重要细胞因子。在感染大鼠类圆线虫的小鼠的肠系膜淋巴结中检测到了IL-3 mRNA，而在未感染小鼠的淋巴结中未检测到。在感染和未感染小鼠的IEL中均检测到了IL-3 mRNA，感染小鼠IEL中的表达增加了20倍。总体而言，感染大鼠类圆线虫后，IEL中的IL-3 mRNA水平高于肠系膜淋巴结中的水平。还在这两个组织中检测了IL-4、IL-10、干细胞因子（SCF或c-kit配体）和IFN-γ mRNA 的表达。在所检测的任何组织中均未检测到IL-10 mRNA，并且大鼠类圆线虫感染后IFN-γ mRNA水平未改变。在感染小鼠的肠系膜淋巴结中观察到SCF（5倍）和IL-4（20倍）mRNA表达升高。相比之下，未感染和感染动物的IEL中SCF mRNA水平相似，并且在感染小鼠的IEL中仅观察到IL-4 mRNA有适度增加。
