Richter W
Int Arch Allergy Appl Immunol. 1975;48(4):505-12. doi: 10.1159/000231338.
The influence of type and degree of substitution of B 512 dextran fractions on reactivity with rabbit antibodies against unmodified B 512 dextran was studied on heterologous passive cutaneous anaphylaxis in guinea pigs. Low degrees of substitution, i.e. 1 substituent group per 7-30 glucose residues, affected reactivity with anti-dextran only slightly or not at all. This was shown for the following substituents: sulphate, carboxymethyl, phosphate, diethylaminoethyl, hydroxypropyl, butyryl, caprylyl, stearoyl, and fluoresceinoyl groups. With high degress of substitution, i.e. 1-3 substituents per 2 glucose residues, reactivity with anti-B-512-dextran is completely abolished for some substituents, and a new immunological identity conferred on the substituted dextran. Stongly charged groups like sulphate, carboxymethyl and diethylaminoethyl abolish reactivity with anti-B-512-dextran at relatively lower degrees of substitution than more neutral groups like methyl and acetyl. The anti-B-512-dextran represents a specific reagent for alpha-1,6-linked polyglucose, as evidenced by complete cross-reactivity with synthetic linear dextran; its specificity is emphasized by non-reactivity with alpha-1,6-linked synthetic manna, the monomeric residues of the two polymers differing only in position of the C-2 hydroxyl groups.
在豚鼠的异种被动皮肤过敏反应中，研究了B 512葡聚糖级分的取代类型和取代度对其与抗未修饰B 512葡聚糖兔抗体反应性的影响。低取代度，即每7 - 30个葡萄糖残基有1个取代基，对与抗葡聚糖的反应性影响很小或没有影响。以下取代基的情况均如此：硫酸根、羧甲基、磷酸根、二乙氨基乙基、羟丙基、丁酰基、辛酰基、硬脂酰基和荧光素酰基。高取代度，即每2个葡萄糖残基有1 - 3个取代基时，某些取代基会使与抗B - 512 - 葡聚糖的反应性完全丧失，并赋予取代葡聚糖一种新的免疫同一性。像硫酸根、羧甲基和二乙氨基乙基这样带强电荷的基团，比甲基和乙酰基等更中性的基团在相对较低的取代度时就会消除与抗B - 512 - 葡聚糖的反应性。抗B - 512 - 葡聚糖是α - 1,6 - 连接的聚葡萄糖的特异性试剂，与合成线性葡聚糖的完全交叉反应证明了这一点；其特异性通过与α - 1,6 - 连接的合成甘露聚糖无反应性得到强调，这两种聚合物的单体残基仅在C - 2羟基的位置上有所不同。
