培养的牛蛙交感神经元中一种毒蕈碱激活的非电压依赖性钾通道。

A muscarine-activated voltage-independent K+ channel in cultured bullfrog sympathetic neurones.

作者信息

Tanaka K, Koyano K, Kuba K

机构信息

Department of Physiology, Saga Medical School, Japan.

出版信息

Neurosci Lett. 1991 Jan 2;121(1-2):191-3. doi: 10.1016/0304-3940(91)90682-j.

DOI:10.1016/0304-3940(91)90682-j

PMID:2020374

Abstract

In cultured bullfrog sympathetic neurones, cell-attached patch clamp revealed a voltage-independent K+ channel having a conductance of 46-113 pS at an external K+ of 2-120 mM. Muscarine (10-20 microM), applied to the cell membrane outside a recording pipette, increased its open probability and mean open time in an atropine-sensitive manner. This muscarine-activated K+ channel could underlie some of the muscarinic inhibitory postsynaptic potentials in both central and peripheral nervous systems.

摘要

在培养的牛蛙交感神经元中，细胞贴附式膜片钳记录显示，在细胞外钾离子浓度为2 - 120 mM时，存在一种非电压依赖性钾离子通道，其电导为46 - 113 pS。将毒蕈碱（10 - 20 microM）施加于记录微电极外的细胞膜上，可使该通道的开放概率和平均开放时间以阿托品敏感的方式增加。这种毒蕈碱激活的钾离子通道可能是中枢和外周神经系统中某些毒蕈碱抑制性突触后电位的基础。