Deubelbeiss K A, Dancey J T, Harker L A, Finch C A
J Clin Invest. 1975 Apr;55(4):833-9. doi: 10.1172/JCI107994.
The production of neutrophils in dogs has been estimated from the number of postmitotic neutrophils in the marrow and the transit time of a [3H]-thymidine pulse. The number of postmitotic neutrophils was derived from the erythron iron turnover measurement of erythroid number and the neutrophil-erythroid ratio in bone marrow sections. The mean value for marrow postmitotic neutrophils in dogs was 5.61 plus or minus 0.56 times 10-9 cells/kg. The mean transit time of these neutrophils was calculated to be 82.1 h. A marrow production of 1.65 times 10-9 neutrophils/kg/day was calculated from these data. The turnover of circulating neutrophils was measured by [3H]thymidine and [32P]diisopropylphospho-fluoridate (DF32P) labeling of blood neutrophils. [3H]-Thymidine labeling gave a calculated recovery of 65 per cent, a t1/2 disappearance time of 6.7 h, and a calculated turnover of 1.66 times 10-9 cells/kg/day. Corresponding results with DF32P tagging were 51 per cent, 5.4 h, and 2.89 times 10-9 cells/kg/day. The discrepancy between these two tags persisted in doubly tagged cells and was considered to be due to elution of DF32P.
通过骨髓中处于有丝分裂后期的中性粒细胞数量以及[³H] - 胸腺嘧啶脉冲的转运时间，对犬类中性粒细胞的生成情况进行了估算。处于有丝分裂后期的中性粒细胞数量是根据红系铁周转率对红系数量的测量结果以及骨髓切片中的中性粒细胞与红细胞比例得出的。犬类骨髓中处于有丝分裂后期的中性粒细胞的平均值为5.61±0.56×10⁻⁹个细胞/千克。这些中性粒细胞的平均转运时间经计算为82.1小时。根据这些数据计算得出骨髓中性粒细胞生成量为1.65×10⁻⁹个中性粒细胞/千克/天。通过用[³H]胸腺嘧啶和[³²P]二异丙基磷酰氟化物（DF³²P）标记血液中的中性粒细胞来测量循环中性粒细胞的周转率。[³H] - 胸腺嘧啶标记得出的计算回收率为65%，半衰期消失时间为6.7小时，计算得出的周转率为1.66×10⁻⁹个细胞/千克/天。DF³²P标记的相应结果分别为51%、5.4小时和2.89×10⁻⁹个细胞/千克/天。这两种标记方法之间的差异在双重标记的细胞中依然存在，被认为是由于DF³²P的洗脱所致。
