Price T H, Dale D C
J Clin Invest. 1977 Mar;59(3):475-80. doi: 10.1172/JCI108662.
A rabbit model was developed to study the in vivo viability of neutrophils stored in vitro for up to 72 h. Acid-citrate-dextrose anticoagulated whole blood was obtained from rabbits previously injected with tritiated thymidine ([3H]thymidine), stored under varying conditions, and then injected into recipient rabbits. Neutrophil viability and function were assessed by measuring the ability of the tagged neutrophils to circulate and to migrate into subcutaneous polyvinyl sponges. Unstored neutrophils disappeared exponentially from the circulation with a t1/2 of 3.2 h and gave a zero time recovery of 30.5%. Storage of cells at either room temperature or 4 degrees C for 24 h or longer resulted in temporary sequestration of cells from active circulation. With cells stored for up to 72 h at 4 degrees C, recovery returned to normal values after 1-2 h. Room temperature stored cells, in contrast, showed evidence of irreversible damage at 48-h storage with low recovery for the entire time span studied. With unstored blood, 8.1+/-0.9% of the injected neutrophil label was present in the subcutaneous sponges. The accumulated label progressively decreased as cell storage time increased reaching at 72 h 5.1+/-0.6 and 2.6+/-0.3% for 4 degrees C and room temperature-stored cells, respectively. The results of this study indicate that 4 degrees C storage of rabbit neutrophils is superior to storage at room temperature. The data suggest that it may be feasible to store neutrophils at least a few days without loss of in vivo functions.
建立了一个兔模型来研究体外储存长达72小时的中性粒细胞的体内活力。从先前注射过氚标记胸腺嘧啶核苷（[³H]胸腺嘧啶核苷）的兔子身上获取酸-柠檬酸-葡萄糖抗凝全血，在不同条件下储存，然后注入受体兔子体内。通过测量标记的中性粒细胞循环和迁移到皮下聚乙烯海绵中的能力来评估中性粒细胞的活力和功能。未储存的中性粒细胞从循环中呈指数级消失，半衰期为3.2小时，零时间回收率为30.5%。在室温或4℃下储存细胞24小时或更长时间会导致细胞暂时从活跃循环中隔离。在4℃下储存长达72小时的细胞，1-2小时后回收率恢复到正常值。相比之下，室温储存的细胞在储存48小时后显示出不可逆损伤的迹象，在整个研究时间段内回收率都很低。对于未储存的血液，8.1±0.9%的注入中性粒细胞标记物存在于皮下海绵中。随着细胞储存时间的增加，积累的标记物逐渐减少，在72小时时，4℃和室温储存的细胞分别为5.1±0.6%和2.6±0.3%。本研究结果表明，兔中性粒细胞在4℃下储存优于在室温下储存。数据表明，储存中性粒细胞至少几天而不丧失体内功能可能是可行的。
