Sever S, Damke H, Schmid S L
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, and Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
Traffic. 2000 May;1(5):385-92. doi: 10.1034/j.1600-0854.2000.010503.x.
The GTPase dynamin is essential for clathrin-mediated endocytosis. Numerous new and exciting discoveries regarding dynamin function in vivo and in vitro have led to various models in which dynamin functions directly in membrane fission and the release of clathrin-coated vesicles from the plasma membrane. This would make dynamin unique among GTPases in its ability to act as a mechanochemical enzyme. Here we review the various models and their supporting data. We then discuss new findings that raise doubts as to whether dynamin breaks the paradigm that governs regulatory GTPases.
GTP酶发动蛋白对于网格蛋白介导的内吞作用至关重要。关于发动蛋白在体内和体外功能的众多新的、令人兴奋的发现，催生了各种模型，在这些模型中，发动蛋白直接参与膜裂变以及网格蛋白包被小泡从质膜的释放。这将使发动蛋白在GTP酶中独树一帜，具备充当一种机械化学酶的能力。在此，我们综述各种模型及其支持数据。然后，我们讨论一些新发现，这些发现让人质疑发动蛋白是否打破了支配调节性GTP酶的范式。
