Heuser J
Department of Cell Biology, Washington University School of Medicine, St. Louis, MO, USA.
Traffic. 2000 Aug;1(8):614-21. doi: 10.1034/j.1600-0854.2000.010805.x.
Today, electron microscopy (EM) is increasingly confronted by the revolution in image-processing technology provoked by modern computers. Digital cameras are fast replacing film-based cameras in EM, as elsewhere, and the procedures for digital image-archiving, image-analysis, and image publication are rapidly evolving. To take advantage of these advances, we have chosen for the moment a 'middle road', in which film remains our basic recording medium in the electron microscope, but immediately thereafter, all film-based images are converted to digital files for further analysis and processing. The rationale behind this approach is that film still offers far greater sensitivity and resolution (providing an image equivalent to > 10,000 pixels per inch in a 1-s exposure), and film is still far easier to organize and archive than digital images of comparable resolution. However, digital manipulation of EM images has become mandatory. Hence, we explain here, in some detail, how we convert from film to digital.
如今，电子显微镜（EM）越来越多地面临着现代计算机引发的图像处理技术革命。与其他领域一样，数码相机正在迅速取代电子显微镜中基于胶片的相机，并且数字图像存档、图像分析和图像发布的程序也在迅速发展。为了利用这些进步，我们目前选择了一条“中间道路”，即胶片仍然是我们电子显微镜中的基本记录介质，但在此之后，所有基于胶片的图像都会立即转换为数字文件，以便进行进一步分析和处理。这种方法背后的基本原理是，胶片仍然具有更高的灵敏度和分辨率（在1秒曝光下可提供相当于每英寸超过10000像素的图像），并且与具有可比分辨率的数字图像相比，胶片仍然更容易整理和存档。然而，对电子显微镜图像进行数字处理已成为必需。因此，我们在此详细解释如何从胶片转换为数字格式。
