原发性胆汁性肝硬化和慢性肝炎中通过超速离心法评估的血浆脂蛋白成分的比较分析。

Comparative analysis of plasma lipoprotein components assessed by ultracentrifugation in primary biliary cirrhosis and chronic hepatitis.

作者信息

Tacikowski T, Milewski B, Dzieniszewski J, Nowicka G, Walewska-Zielecka B

机构信息

Clinic of Metabolic Diseases and Gastroenterology, National Institute of Food and Nutrition, ul. Kondratowicza 8, 03-242 Warsaw, Poland.

出版信息

Med Sci Monit. 2000 Mar-Apr;6(2):325-9.

PMID:11208331

Abstract

UNLABELLED

The evaluation of lipid and lipoprotein profiles in liver diseases has important cognitive aspects and provides practical information contributing to the diagnosis of liver pathology. There are few studies of this problem using the ultracentrifugation method.

AIM OF THE STUDY

A comparison of lipid profile (obtained by plasma ultracentrifugation) in patients with primary biliary cirrhosis (PBC) and chronic hepatitis (CH).

MATERIAL AND METHODS

103 percutaneous liver biopsies were routinely performed from 1994 to 1997. Blood samples were taken from all the patients at the time of biopsy for further evaluation of lipid profile. 15 patients with PBC and 15 patients with CH (of HBV or/and HCV etiology) were studied.

RESULTS

In patients with CH mean total, esterified and free cholesterol levels (166 mg%, 117.6 mg% and 48.1 mg%, respectively) were significantly lower (p < 0.002; p < 0.004; p < 0.006, respectively) than in patients with PBC (237.5 mg%, 165.7 mg% and 71.8 mg%, respectively). The phospholipid concentration in sera of patients with PBC were significantly higher (271.1 mg%, p < 0.0004) than in patients with CH (187.6 mg%), whereas apolipoprotein B and apoAII were significantly lower. Total, esterified and free cholesterol levels in LDL fraction were significantly higher in patients with PBC (175.3 mg%, p < 0.007; 117.9 mg%, p < 0.02; 57.6 mg%, p < 0.01, respectively) than in patients with CH (113.7 mg%, 78.7 mg% and 35 mg%, respectively). The concentration of phospholipids in LDL fraction in patients with PBC was significantly higher (166.8 mg%; p < 0.003) in comparison with patients with CH (96.3 mg%). The differences in other lipoprotein fractions (VLDL and HDL) were not statistically significant.

CONCLUSIONS

High levels of total, free and esterified cholesterol as well as phospholipids, apolipoprotein B and AII were observed by us in patients with PBC in comparison with patients suffering from CH. The increase of cholesterol (total, esterified and free) and the phospholipid concentration in serum, are a manifestation of their higher concentration in LDL fraction.

摘要

未标记

评估肝脏疾病中的脂质和脂蛋白谱具有重要的认知意义，并为肝脏病理学诊断提供实用信息。使用超速离心法对该问题进行研究的较少。

研究目的

比较原发性胆汁性肝硬化（PBC）患者和慢性肝炎（CH）患者的脂质谱（通过血浆超速离心获得）。

材料与方法

1994年至1997年常规进行了103例经皮肝活检。在活检时采集所有患者的血样以进一步评估脂质谱。研究了15例PBC患者和15例CH患者（病因是HBV或/和HCV）。

结果

CH患者的平均总胆固醇、酯化胆固醇和游离胆固醇水平（分别为166mg%、117.6mg%和48.1mg%）显著低于PBC患者（分别为237.5mg%、165.7mg%和71.8mg%）（分别p<0.002；p<0.004；p<0.006）。PBC患者血清中的磷脂浓度显著高于CH患者（271.1mg%，p<0.0004）（187.6mg%），而载脂蛋白B和载脂蛋白AII显著更低。PBC患者LDL组分中的总胆固醇、酯化胆固醇和游离胆固醇水平显著高于CH患者（分别为175.3mg%，p<0.007；117.9mg%，p<0.02；57.6mg%，p<0.01）（分别为113.7mg%、78.7mg%和35mg%）。与CH患者（96.3mg%）相比，PBC患者LDL组分中的磷脂浓度显著更高（166.8mg%；p<0.003）。其他脂蛋白组分（VLDL和HDL）的差异无统计学意义。