Kilic Suleyman Sirri, Aydin Suleyman, Kilic Nermin, Erman Fazilet, Aydin Suna, Celik Ilhami
Department of Biochemistry and Clinical Biochemistry, Medical School (Firat Medical Center), Firat University, Elazig 23119, Turkey.
World J Gastroenterol. 2005 Dec 14;11(46):7351-4. doi: 10.3748/wjg.v11.i46.7351.
To investigate the relationship between serum paraoxonase (PON1), AST, ALT, GGT, and arylesterase (AE) activity alterations and the degree of liver damage in patients with chronic hepatitis.
We studied 34 chronic hepatitis patients and 32 control subjects, aged between 35 and 65 years, in the Department of Infection and Clinical Microbiology at the Firat University School of Medicine. Blood samples were collected from subjects between 8:00 and 10:00 a.m. following a 12-h fast. Baseline and salt-stimulated PON1 activities were measured by the hydrolysis of paraoxon. Phenyl acetate was used as the substrate and formed phenol was measured spectrophotometrically at 270 nm after the addition of a 10-fold diluted serum sample in AE activity measurements.
The results of this investigation revealed that the levels of AE activity decreased from 132+/-52 to 94+/-36 (29%), baseline PON1 activity from 452+/-112 to 164+/-67 (64%), salt-stimulated PON1 activity from 746+/-394 to 294+/-220 (61%), HDL from 58.4+/-5.1 to 47.2+/-5.6 (20%), triglyceride from 133+/-51.2 to 86+/-34.0 (35%), while a slight increase in the level of LDL (from 163+/-54.1 to 177.3+/-56.0; 9%) and significant increases in the levels of AST (from 29+/-9.3 to 98+/-44), ALP (from 57.2+/-13.1 to 91+/-38.1), ALT (from 27.9+/-3.32 to 89+/-19.1), GGT (from 24.3+/-2.10 to 94+/-48.2), total bilirubin (from 0.74+/-0.02 to 1.36+/-0.06; 84%) and direct bilirubin (from 0.18+/-0.01 to 0.42+/-0.04; 133%) were detected. However, the levels of albumin, total protein, cholesterol, and uric acid were almost the same in chronic hepatitis and the control subjects.
Low PON1 and AE activity may contribute to the increased liver dysfunction in chronic hepatitis patients by reducing the ability of HDL to retard LDL oxidation and might be clinically useful for monitoring the disease of chronic hepatitis.
探讨慢性肝炎患者血清对氧磷酶（PON1）、天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、γ-谷氨酰转肽酶（GGT）及芳基酯酶（AE）活性变化与肝损伤程度的关系。
我们对菲拉特大学医学院感染与临床微生物学系34例慢性肝炎患者和32例年龄在35至65岁之间的对照者进行了研究。上午8:00至10:00之间，在受试者禁食12小时后采集血样。通过对氧磷水解法测定基线和盐刺激后的PON1活性。在AE活性测定中，以乙酸苯酯为底物，加入10倍稀释的血清样品后，在270nm处用分光光度法测定生成的苯酚。
本研究结果显示，AE活性水平从132±52降至94±36（29%），基线PON1活性从452±112降至164±67（64%），盐刺激后的PON1活性从746±394降至294±220（61%），高密度脂蛋白（HDL）从58.4±5.1降至47.2±5.6（20%），甘油三酯从133±51.2降至86±34.0（35%），而低密度脂蛋白（LDL）水平略有升高（从163±54.1升至177.3±56.0；9%），AST（从29±9.3升至98±44）、碱性磷酸酶（ALP，从57.2±13.1升至91±38.1）、ALT（从27.9±3.32升至89±19.1）、GGT（从24.3±2.10升至94±48.2）、总胆红素（从0.74±0.02升至1.36±0.06；84%）和直接胆红素（从0.18±0.01升至0.42±0.04；133%）水平显著升高。然而，慢性肝炎患者和对照者的白蛋白、总蛋白、胆固醇和尿酸水平几乎相同。
低PON1和AE活性可能通过降低HDL抑制LDL氧化的能力，导致慢性肝炎患者肝功能障碍加重，可能对监测慢性肝炎病情具有临床意义。
