Bergman R, Malkin L, Sabo E, Kerner H
Department of Dermatology, Rambam Medical Center, Technion-Israel Institute of Technology, Haifa, Israel.
J Am Acad Dermatol. 2001 Mar;44(3):500-4. doi: 10.1067/mjd.2001.111635.
The histopathologic differential diagnosis of Spitz nevus (SN) from malignant melanoma (MM) may be difficult.
Our purpose was to determine the staining pattern and usefulness of MIB-1 antibody, which recognizes Ki-67 antigen in formalin-fixed, paraffin-embedded tissue, as an adjunct to the histopathologic differential diagnosis of SN.
Twenty-five compound SNs, 27 MMs, and 26 compound nondysplastic melanocytic nevi (MNs) were immunostained with the MIB-1 antibody.
The mean counts of MIB-1--stained tumor cells of the epidermal and dermal components, both alone and together, were significantly lower in SNs and MNs than in MMs (P <.0001). The dermal counts showed the best discriminating power. In addition, the mean dermal/epidermal count ratios for MIB-1 in SNs and MNs (0.25 and 0.23, respectively) were significantly lower than the corresponding ratio (0.94) in MMs (P <.0001).
MIB-1-stained tumor cell counts, especially of the dermal component, and dermal/epidermal MIB-1 count ratios may be helpful as an adjunct to the histopathologic differential diagnosis of SN.
Spitz痣（SN）与恶性黑色素瘤（MM）的组织病理学鉴别诊断可能存在困难。
我们的目的是确定MIB-1抗体在福尔马林固定、石蜡包埋组织中识别Ki-67抗原的染色模式及效用，以辅助SN的组织病理学鉴别诊断。
对25例复合性SN、27例MM和26例复合性非发育异常性黑素细胞痣（MN）进行MIB-1抗体免疫染色。
SN和MN的表皮及真皮成分中，单独及合并的MIB-1染色肿瘤细胞平均计数均显著低于MM（P<.0001）。真皮计数显示出最佳鉴别能力。此外，SN和MN中MIB-1的平均真皮/表皮计数比值（分别为0.25和0.23）显著低于MM中的相应比值（0.94）（P<.0001）。
MIB-1染色肿瘤细胞计数，尤其是真皮成分的计数以及真皮/表皮MIB-1计数比值，可能有助于辅助SN的组织病理学鉴别诊断。
