Castells C B, Carr P W
Department of Chemistry, University of Minnesota, Minneapolis 55455, USA.
J Chromatogr A. 2000 Dec 22;904(1):17-33. doi: 10.1016/s0021-9673(00)00883-9.
In this work, we study the influence of the mobile phase and column temperature on the enantioresolution of basic compounds on microparticulate porous zirconia coated with cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC). The chiral analytes are amino compounds, including a number of beta-blockers. Analytes are eluted with hexane-alcohol mobile phases. We investigated the effect of alcohol (type and concentration), basic eluent additives, and column temperature on the parameters that control resolution (column efficiency, retention and selectivity). Conditions for achieving an adequate separation in the least time have been determined for numerous racemic mixtures. For most solutes, baseline resolution of the enantiomeric pair was achieved in less than 1 min; 12 of 13 pairs were separated in less than 2 min.
在本研究中，我们考察了流动相和柱温对涂覆三（3,5-二甲基苯基氨基甲酸酯）纤维素（CDMPC）的微粒多孔氧化锆上碱性化合物对映体拆分的影响。手性分析物为氨基化合物，包括多种β-阻滞剂。分析物用己烷-醇流动相洗脱。我们研究了醇（类型和浓度）、碱性洗脱添加剂和柱温对控制拆分的参数（柱效、保留和选择性）的影响。已确定了众多外消旋混合物在最短时间内实现充分分离的条件。对于大多数溶质，对映体对在不到1分钟内实现基线分离；13对中的12对在不到2分钟内分离。
