Aoyama T, Fukui K, Nakamori T, Hashimoto Y, Yamamoto T, Takamatsu K, Sugano M
Novelty Materials Research Institute, Hannan R&D Center, Fuji Oil Co. Ltd., Izumisano, Osaka, Japan.
Biosci Biotechnol Biochem. 2000 Dec;64(12):2594-600. doi: 10.1271/bbb.64.2594.
The effect on genetically obese mice of a milk whey protein isolate (WPI) and soy protein isolate (SPI) and their hydrolysates (WPI-H, SPI-H) on the rate of body fat disappearance was investigated. Male yellow KK mice were made obese by feeding with a high-fat diet containing 30% fat from 6 to 10 weeks of age. They were then fed with an energy-restricted low fat (5.0%) and high protein (35% WPI, WPI-H, SPI or SPI-H) diet for 2 weeks at the 60% level of energy intake by mice on laboratory feed. During the weight reduction period, the body weight of the WPI, WPI-H, SPI and SPI-H groups changed by -9.1, -9.1, -10.0 and -11.1 g/14 days, respectively, the reduction being significantly lower in the SPI-H group than in the WPI and WPI-H groups. The plasma total cholesterol level was significantly lower with the SPI diet, and the plasma glucose level was lower with the SPI and SPI-H diets than with the WPI and WPI-H diets. Although the body protein content was comparable in all the groups, the body fat content was significantly lower with the SPI diet than with the WPI diet, and was also significantly lower with the SPI-H diet than with the WPI and WPI-H diets. The weight of the perirenal fat pads was significantly lower with the SPI-H diet than with the WPI and WPI-H diets. These results indicate that SPI and SPI-H are suitable protein sources in an energy-restricted diet for treating obesity.
研究了乳清蛋白分离物（WPI）和大豆蛋白分离物（SPI）及其水解产物（WPI-H、SPI-H）对遗传性肥胖小鼠体脂消失率的影响。雄性黄色KK小鼠在6至10周龄时通过喂食含30%脂肪的高脂饮食而变得肥胖。然后，按照实验室饲料喂养的小鼠能量摄入量的60%水平，给它们喂食能量受限的低脂（5.0%）和高蛋白（35% WPI、WPI-H、SPI或SPI-H）饮食2周。在体重减轻期间，WPI、WPI-H、SPI和SPI-H组的体重分别每14天变化-9.1、-9.1、-10.0和-11.1 g，SPI-H组的体重减轻明显低于WPI和WPI-H组。SPI饮食组的血浆总胆固醇水平显著较低，SPI和SPI-H饮食组的血浆葡萄糖水平低于WPI和WPI-H饮食组。尽管所有组的身体蛋白质含量相当，但SPI饮食组的体脂含量显著低于WPI饮食组，SPI-H饮食组的体脂含量也显著低于WPI和WPI-H饮食组。SPI-H饮食组的肾周脂肪垫重量显著低于WPI和WPI-H饮食组。这些结果表明，SPI和SPI-H是能量受限饮食中治疗肥胖的合适蛋白质来源。
