Akahoshi Asuka, Koba Kazunori, Enmoto Rie, Nishimura Kazuko, Honda Yukiko, Minami Miyuki, Yamamoto Keiko, Iwata Toshio, Yamauchi Yoshie, Tsutsumi Kentaro, Sugano Michihiro
Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Japan.
Biosci Biotechnol Biochem. 2005 Dec;69(12):2409-15. doi: 10.1271/bbb.69.2409.
The interaction of dietary protein type and fat level on the body fat-reducing activity of conjugated linoleic acid (CLA) was studied in male rats fed diets containing casein (CAS) or soy protein (SOY) as a protein source with low fat (LF, 6.0% soybean oil) or high fat (HF, 13.0% soybean oil) combinations for 4 weeks. CLA was added at the 1.0% level to all diets. The weight of perirenal adipose tissue tended to be lower in the SOY groups than in the corresponding CAS groups, and the difference between the LF diets was significant. The weight of epididymal adipose tissue showed a similar but insignificant trend. The weight of brown adipose tissue was heaviest on the SOY-HF diet and lowest on two CAS diets, the SOY-LF diet being intermediate. The concentration of serum leptin was lowest on the SOY-LF diet and was significantly lower than that of the corresponding CAS group, but this difference disappeared when the dietary fat level increased. The serum cholesterol-lowering activity of SOY in relation to CAS was reproduced even when CLA was given. Thus the body fat-reducing activity of CLA was most marked when rats were fed the SOY-LF diet. Although the CAS-HF diet increased body fat deposition, the magnitude of the reduction by lowering dietary fat level was more marked than in the case of SOY. These results indicate a complicated interaction of dietary manipulations with the body fat-reducing effect of CLA, but the combination of CLA with the SOY-LF diet appears to be an appropriate approach.
在雄性大鼠中研究了膳食蛋白质类型和脂肪水平对共轭亚油酸（CLA）减脂活性的相互作用。给雄性大鼠喂食以酪蛋白（CAS）或大豆蛋白（SOY）作为蛋白质来源，搭配低脂（LF，6.0%大豆油）或高脂（HF，13.0%大豆油）组合的饲料，持续4周。所有饲料中CLA的添加水平均为1.0%。大豆蛋白组大鼠的肾周脂肪组织重量往往低于相应的酪蛋白组，低脂饲料组之间的差异具有显著性。附睾脂肪组织重量呈现类似趋势，但差异不显著。棕色脂肪组织重量在大豆蛋白-高脂饲料组中最重，在两种酪蛋白饲料组中最轻，大豆蛋白-低脂饲料组居中。血清瘦素浓度在大豆蛋白-低脂饲料组中最低，且显著低于相应的酪蛋白组，但当膳食脂肪水平升高时，这种差异消失。即使给予CLA，大豆蛋白相对于酪蛋白的降血清胆固醇活性依然存在。因此，当给大鼠喂食大豆蛋白-低脂饲料时CLA的减脂活性最为显著。虽然酪蛋白-高脂饲料增加了体脂沉积，但降低膳食脂肪水平时体脂减少的幅度比大豆蛋白组更为明显。这些结果表明膳食调控与CLA的减脂作用之间存在复杂的相互作用，但CLA与大豆蛋白-低脂饲料的组合似乎是一种合适的方法。
