Pilvi Taru K, Harala Saara, Korpela Riitta, Mervaala Eero M
Institute of Biomedicine, Pharmacology, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland.
Br J Nutr. 2009 Aug;102(3):337-41. doi: 10.1017/S0007114508199445.
The aim of the study was to compare the effect of different whey protein-containing high-Ca diets on weight loss and weight regain in a model of diet-induced obesity. Obesity was induced in C57BL/6J mice with a high-fat (60 % of energy) diet. Weight loss by energy restriction was performed on four different high-Ca diets (1.8 % CaCO3) containing different whey proteins (18 % of energy): alpha-lactalbumin (ALA), beta-lactoglobulin (BLG), lactoferrin (LF) and whey protein isolate (WPI). After 7 weeks of energy restriction some of the mice were killed and the rest were fed with the same diets ad libitum for 7 weeks. The mice on the LF diet lost significantly more weight than mice on the WPI diet. The body fat content in the ALA and LF groups was significantly lower than in the WPI group (P < 0.05) and the LF group differed significantly even from the BLG group (P < 0.05). Ad libitum feeding after weight loss resulted in weight regain in all groups and only the ALA diet significantly reduced fat accumulation during weight regain. The weight regain was most pronounced in the LF group, but the adipocyte size was still significantly smaller than in the other groups. There were no differences in food intake or apparent fat digestibility between the groups. It can be concluded that a high-Ca diet with ALA significantly improves the outcome of weight loss and subsequent weight regain during the feeding of a high-fat diet in C57BL/6J mice, in comparison with WPI.
本研究的目的是在饮食诱导肥胖模型中，比较不同含乳清蛋白的高钙饮食对体重减轻和体重反弹的影响。用高脂（占能量的60%）饮食诱导C57BL/6J小鼠肥胖。通过能量限制在四种不同的高钙饮食（1.8%碳酸钙）上进行体重减轻，这些饮食含有不同的乳清蛋白（占能量的18%）：α-乳白蛋白（ALA）、β-乳球蛋白（BLG）、乳铁蛋白（LF）和乳清蛋白分离物（WPI）。能量限制7周后，处死部分小鼠，其余小鼠随意进食相同饮食7周。LF饮食组的小鼠比WPI饮食组的小鼠体重减轻明显更多。ALA组和LF组的体脂含量显著低于WPI组（P<0.05），LF组甚至与BLG组也有显著差异（P<0.05）。体重减轻后随意进食导致所有组体重反弹，只有ALA饮食在体重反弹期间显著减少脂肪堆积。LF组体重反弹最为明显，但其脂肪细胞大小仍显著小于其他组。各组之间食物摄入量或表观脂肪消化率没有差异。可以得出结论，与WPI相比，含ALA的高钙饮食在C57BL/6J小鼠高脂饮食喂养期间显著改善体重减轻及随后体重反弹的结果。
