Talebi-Ardakani Mohammad Reza, Torshabi Maryam, Karami Elahe, Vajar Nazanin, Rezaei Esfahrood Zeinab, Meimandi Mansour, Mojahedi Seyed Masoud
Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2017 Summer;8(Suppl 1):S51-S55. doi: 10.15171/jlms.2017.s10. Epub 2017 Aug 29.
The present study compared the effects of erbium-doped yttrium aluminium garnet (Er:YAG) laser and hand instrumentation on the attachment of human gingival fibroblast (HGF) cells to periodontally involved root surfaces. A total of 40 tooth specimens were collected and treated in four distinct groups: scaled and root planed with hand instruments, scaled with Er:YAG laser, treated with a combination of hand instruments and Er:YAG laser and non-treated control group. The attachment and proliferation rate of HGF were assessed using MTT assay and scanning electron microscope (SEM) examination was used for cell morphological evaluation. The MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay showed significant decrease in HGF cell viability in both hand instruments only and combination treated teeth specimens compared to control specimens (<0.05), 24 hours after cell seeding. However, at time 48, the cell viability of attached cells in these 2 treated groups was almost similar to control. In contrast, at 24 and 48 hours after cell seeding, viability of attached cells was higher than control in Er:YAG laser treated only specimens (<0.05). According to SEM study, the laser treated specimens showed more surface roughness. Er:YAG laser increased attachment and proliferation of HGF cells in comparison to the hand instruments method.
本研究比较了掺铒钇铝石榴石（Er:YAG）激光和手工器械处理对人牙龈成纤维细胞（HGF）附着于牙周病累及牙根表面的影响。共收集40颗牙齿标本，分为四组进行不同处理：用手工器械进行刮治和根面平整、用Er:YAG激光进行刮治、联合使用手工器械和Er:YAG激光进行处理以及未处理的对照组。采用MTT法评估HGF的附着和增殖率，并用扫描电子显微镜（SEM）检查进行细胞形态学评估。MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑）法显示，在细胞接种24小时后，仅使用手工器械处理组和联合处理组的牙齿标本中，HGF细胞活力与对照标本相比显著降低（<0.05）。然而，在48小时时，这两个处理组中附着细胞的活力与对照几乎相似。相比之下，在细胞接种24小时和48小时后，仅用Er:YAG激光处理的标本中附着细胞的活力高于对照（<0.05）。根据SEM研究，激光处理的标本表面粗糙度更高。与手工器械方法相比，Er:YAG激光可增加HGF细胞的附着和增殖。
