Naughton Patrick J, Grant George, Bardocz Susan, Allen-Vercoe Emma, Woodward Martin J, Pusztai Arpad
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB and *Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey KT15 3NB.
J Med Microbiol. 2001 Feb;50(2):191-197. doi: 10.1099/0022-1317-50-2-191.
The involvement of type 1 fimbriae in colonisation of the rat gastrointestinal tract in vivo was investigated with Salmonella enterica serotype Enteritidis LA5 and a mutant of LA5 denoted EAV3 unable to elaborate type 1 fimbriae (SEF 21). Rats were given a single dose of LA5 or EAV3 or a 1:1 mixture of both. LA5 was found in higher numbers in the stomach and small intestine than EAV3 at 6 h after infection with a single strain, but not after 6 days. LA5 did not out-compete EAV3 when the strains were administered together. Indeed, after 6 and 21 days, EAV3 was found in the distal small intestine and large intestine in far higher numbers than LA5. These findings suggest that SEF 21 have an important role(s) in the early stages of infection in vivo. However, SEF 21 expression may disadvantage the pathogen in the longer term as indicated by EAV3 out-competing LA5 in the gut at 21 days.
利用肠炎沙门氏菌肠炎血清型LA5和LA5的一个突变体EAV3（无法产生1型菌毛（SEF 21）），研究了1型菌毛在大鼠胃肠道体内定植中的作用。给大鼠单剂量注射LA5或EAV3或两者的1:1混合物。在单菌株感染后6小时，胃和小肠中LA5的数量高于EAV3，但6天后并非如此。当一起给药时，LA5并没有胜过EAV3。事实上，在6天和21天后，在远端小肠和大肠中发现EAV3的数量远远高于LA5。这些发现表明，SEF 21在体内感染的早期阶段具有重要作用。然而，如EAV3在21天时在肠道中胜过LA5所示，从长远来看，SEF 21的表达可能对病原体不利。
