Robertson W J, Bowman J P, Franzmann P D, Mee B J
Centre for Groundwater Studies, CSIRO Land and Water, Floreat Park, WA, Australia.
Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):133-40. doi: 10.1099/00207713-51-1-133.
Eight strains of spore-forming, sulfate-reducing bacteria, isolated from groundwater contaminated with motor fuel [mostly benzene, toluene ethylbenzene and xylene (BTEX) compounds] in sandy soil near Perth, Australia, were closely related to Desulfosporosinus (previously Desulfotomaculum) orientis DSM 765T (95.3-97.3% 16S rDNA sequence similarity). Whole-cell fatty acids were dominated by even-carbon, straight-chain saturated and mono-unsaturated fatty acids, in particular 16:0, 16:1cis9, 14:0 and 18:1cis11. The strains grew at temperatures between 4 and 42 degrees C and in medium containing up to 4% NaCl. The eight strains clustered into two main groups based on phylogeny, randomly amplified polymorphic DNA (RAPD)-PCR patterns and nutritional characteristics. Representatives of the two groups, strain S5 (group A) and strain S10T (group B) had 81% DNA-DNA homology with each other and therefore should be accommodated in the same species. Strain S10T had less than 38% homology with Desulfosporosinus orientis DSM 765T, the most closely phylogenetically related type strain available. The new strains were distinguished from Desulfosporosinus orientis DSM 765T by different banding patterns in a RAPD-PCR, and phenotypically by their inability to utilize fumarate as a carbon and energy source with sulfate as the electron acceptor and by their lower tolerance to NaCl. The DNA G+C contents were 46.8 and 46.9 mol% for strains S5 and S10T, respectively (Desulfosporosinus orientis DSM 765T 45.9 mol%). It is proposed that these new strains be placed in a new species of the genus Desulfosporosinus. The name Desulfosporosinus meridiei is proposed, with strain S10T as the type strain (= DSM 13257T = NCIMB 13706T).
从澳大利亚珀斯附近沙地中受汽车燃料污染（主要是苯、甲苯、乙苯和二甲苯（BTEX）化合物）的地下水中分离出8株产芽孢、硫酸盐还原菌，它们与东方脱硫孢菌（以前的东方脱硫肠状菌）DSM 765T密切相关（16S rDNA序列相似性为95.3 - 97.3%）。全细胞脂肪酸以偶数碳、直链饱和及单不饱和脂肪酸为主，尤其是16:0、16:1cis9、14:0和18:1cis11。这些菌株在4至42摄氏度的温度范围内以及含高达4%氯化钠的培养基中生长。基于系统发育、随机扩增多态性DNA（RAPD）-PCR图谱和营养特征，这8株菌聚为两个主要类群。两个类群的代表菌株S5（A组）和菌株S10T（B组）彼此间的DNA - DNA同源性为81%，因此应归为同一物种。菌株S10T与系统发育关系最密切的现有模式菌株东方脱硫孢菌DSM 765T的同源性低于38%。新菌株与东方脱硫孢菌DSM 765T的区别在于RAPD - PCR中的条带模式不同，以及表型上不能以延胡索酸为碳源和能源、以硫酸盐为电子受体，且对氯化钠的耐受性较低。菌株S5和S10T的DNA G + C含量分别为46.8和46.9 mol%（东方脱硫孢菌DSM 765T为45.9 mol%）。建议将这些新菌株归入脱硫孢菌属的一个新物种。提议将其命名为南方脱硫孢菌，菌株S10T为模式菌株（= DSM 13257T = NCIMB 13706T）。
