Van Regenmortel M H
Institut de Biologie Moléculaire et Cellulaire, CNRS, IBMC, Strasbourg, France.
Dev Biol (Basel). 2000;103:69-74.
There is considerable interest in the possibility of using surrogate physico-chemical assays for the quality control of vaccine components. Since biological activity always depends on a first binding step, the primary criterion for assessing biological activity is the ability of a vaccine component to bind specifically in a binding assay. Biosensor instruments that measure binding kinetics with considerable ease and precision are used increasingly to assess the quality of antigens intended for vaccination as well as the binding affinity of antibodies elicited by vaccination. The most widely used biosensor instrument is the BIACORE and its mode of operation is briefly described. The use of biosensors for measuring the active concentration of biomolecules and for assessing the probable effectiveness of vaccine-induced antibodies is described.
人们对使用替代物理化学分析方法进行疫苗成分质量控制的可能性非常感兴趣。由于生物活性总是依赖于最初的结合步骤，评估生物活性的主要标准是疫苗成分在结合分析中特异性结合的能力。能够相当轻松且精确地测量结合动力学的生物传感器仪器越来越多地用于评估用于疫苗接种的抗原质量以及疫苗接种所引发抗体的结合亲和力。使用最广泛的生物传感器仪器是BIACORE，其操作模式将简要介绍。本文还描述了使用生物传感器测量生物分子的活性浓度以及评估疫苗诱导抗体可能有效性的方法。
