Shimaoka Y, Hidaka Y, Tada H, Takeoka K, Morimoto Y, Amino N
Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita, Japan.
Am J Reprod Immunol. 2001 Feb;45(2):100-2. doi: 10.1111/j.8755-8920.2001.450206.x.
Recently, we reported increases in the production of interferon-gamma (IFN-gamma), interleukin-2 (IL-2), and IL-4 during the postpartum period. The present study was undertaken to investigate whether these increases might be explained by increased prolactin while breast-feeding.
Whole blood from 41 women who were breast-feeding, 13 women not breast-feeding, and 31 healthy non-pregnant women was stimulated with phorbol 12-myristate 13-acetate and ionomycin, and the levels of cytokines in the supernatant were measured by enzyme-linked immunosorbent assay. Their serum levels of prolactin were measured by enzyme immunoassay.
Increases in IFN-gamma, IL-2, IL-4, and IL-10 production were observed in women who were breast-feeding but not in women who were not breast-feeding. Serum levels of prolactin correlated with the levels of IFN-gamma in culture supernatant.
These results suggest that breast-feeding induces production of cytokines and that IFN-gamma production is enhanced by physiological concentrations of prolactin.
最近，我们报道了产后干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）和IL-4的产生增加。本研究旨在调查这些增加是否可能由母乳喂养期间催乳素增加所解释。
用佛波酯12-肉豆蔻酸酯13-乙酸酯和离子霉素刺激41名母乳喂养女性、13名非母乳喂养女性及31名健康未孕女性的全血，采用酶联免疫吸附测定法测量上清液中细胞因子的水平。采用酶免疫测定法测量她们血清中的催乳素水平。
在母乳喂养的女性中观察到IFN-γ、IL-2、IL-4和IL-10产生增加，但在非母乳喂养的女性中未观察到。血清催乳素水平与培养上清液中IFN-γ水平相关。
这些结果表明母乳喂养可诱导细胞因子的产生，并且生理浓度的催乳素可增强IFN-γ的产生。
