Pochhammer C, Dietsch P, Siegmund P R
J Histochem Cytochem. 1979 Jul;27(7):1103-7. doi: 10.1177/27.7.112177.
A new specific method for the detection of carbonic anhydrase, EC 4.2.1.1, in tissues is described. The reaction of carbonic anhydrase with dimethylaminonaphthalene-5-sulfonamide (DNSA) forms a highly fluorescent complex. The specificity of the method is proved by the quenching of this fluorescence with ethoxzolamide (6-ethoxybenzothiazole-5-sulfonamide). The difference in the wavelength makes it possible to absorb the fluorescence of the unbound dimethylaminonaphthalene-5-sulfonamide by filters. Kidney, proventriculus, and bone from chicken have been examined. Carbonic anhydrase has been detected in the cytoplasm of the columnar lining cells, proximal tubule cells, and osteoclasts.
本文描述了一种检测组织中碳酸酐酶（EC 4.2.1.1）的新的特异性方法。碳酸酐酶与二甲基氨基萘-5-磺酰胺（DNSA）反应形成一种高荧光复合物。用乙氧唑胺（6-乙氧基苯并噻唑-5-磺酰胺）淬灭这种荧光证明了该方法的特异性。波长的差异使得通过滤光片吸收未结合的二甲基氨基萘-5-磺酰胺的荧光成为可能。已对鸡的肾脏、前胃和骨骼进行了检测。在柱状衬里细胞、近端小管细胞和破骨细胞的细胞质中检测到了碳酸酐酶。
