埃坡霉素生物合成的研究：碳骨架的生物合成起源

Studies on the biosynthesis of epothilones: the biosynthetic origin of the carbon skeleton.

作者信息

Gerth K, Steinmetz H, Höfle G, Reichenbach H

机构信息

GBF, Gesellschaft für Biotechnologische Forschung mbH, Abteilung Naturstoffbiologie, Braunschweig, Germany.

出版信息

J Antibiot (Tokyo). 2000 Dec;53(12):1373-7. doi: 10.7164/antibiotics.53.1373.

DOI:10.7164/antibiotics.53.1373

PMID:11217802

Abstract

The biosynthetic origin of the epothilone skeleton was studied by the incorporation of 13C and radioactively labeled precursors by Sorangium cellulosum So ce90. The carbon atoms are derived from acetate, propionate, the methyl group of S-adenosyl-methionine, and cysteine which also introduces the sulfur and nitrogen atoms. Epothilone biosynthesis starts with the formation of the thiazole part from acetate and cysteine. The incorporation of acetate or propionate units results in the formation of epothilones A and B, respectively. To introduce the epoxide function of epothilones A and B molecular oxygen is used.

摘要

通过纤维堆囊菌So ce90掺入13C和放射性标记的前体来研究埃坡霉素骨架的生物合成起源。碳原子来源于乙酸盐、丙酸盐、S-腺苷甲硫氨酸的甲基以及半胱氨酸，半胱氨酸还引入了硫和氮原子。埃坡霉素的生物合成始于由乙酸盐和半胱氨酸形成噻唑部分。乙酸盐或丙酸盐单元的掺入分别导致埃坡霉素A和B的形成。使用分子氧来引入埃坡霉素A和B的环氧官能团。