Padilla J A, Martínez-Trancón M, Rabasco A, Parejo J C, Sansinforiano M E, Guijo M I
Department of Genetic and Animal Breeding, Faculty of Veterinary Studies, University of Extremadura, Spain.
J Hered. 2000 Nov-Dec;91(6):495-9. doi: 10.1093/jhered/91.6.495.
RAPD analysis was used to estimate the genetic diversity in an Iberian imperial eagle (Aquila adalberti) population, one of the most threatened bird species in the world. Forty-five of 60 arbitrarily designed primers amplified 614 loci in 25 individual eagles, 59.7% of which were polymorphic. In contrast to the traditional allozyme analysis performed in a previous study, the RAPD method has revealed a high level of heterozygosity in this species (H = 0.267+/-0.008). The genetic distances estimated between 25 eagles can serve to establish more adequate mating in order to preserve genetic variability. Conservation efforts being carried out in Spain in this species might be successful based on the results obtained in the present work.
随机扩增多态性DNA分析被用于评估伊比利亚帝国鹰（Aquila adalberti）种群的遗传多样性，该种群是世界上最濒危的鸟类之一。60个任意设计的引物中有45个在25只个体鹰中扩增出614个位点，其中59.7%为多态性位点。与先前研究中进行的传统等位酶分析不同，随机扩增多态性DNA方法揭示了该物种的高杂合度水平（H = 0.267±0.008）。估计的25只鹰之间的遗传距离可用于建立更合适的配对，以保护遗传变异性。基于本研究获得的结果，西班牙针对该物种正在开展的保护工作可能会取得成功。
