Tsuzuki S, Ota H, Hayama M, Sugiyama A, Akamatsu T, Kawasaki S
First Dept of Surgery, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Scand J Gastroenterol. 2001 Jan;36(1):86-91. doi: 10.1080/00365520150218101.
The lamina propria of the digestive tract is the space containing vessels, myofibroblasts, and other interstitial components. The present study was undertaken to elucidate the relationships between the proliferation of myofibroblasts within this space and other histological features such as inflammatory cell infiltration and proliferation of blood vessels.
Thirty-eight cases in total-comprising 19 cases of early and 19 cases of advanced esophageal squamous cell carcinoma with intraepithelial extension (the former including 10 lesions of carcinoma in situ and 15 lesions of carcinoma with invasion only into the lamina propria)-were examined using H&E staining, Azan Mallory staining, and immunostaining for the characterization of mesenchymal cells in the lamina propria against alpha-smooth muscle actin (alphaSMA), desmin, vimentin, factor VIII, collagen type IV, laminin, or inflammatory cells (L26, UCHL1, Kp1, and c-kit). The proliferative potential of myofibroblasts was evaluated by measuring the total length of the bundles of myofibroblasts per case.
Proliferation of alphaSMA-containing stromal cells: i) occurred in the lamina propria subjacent either to intraepithelial carcinomas (64%) or to the intraepithelial extension of carcinoma tissue (47%), and ii) showed a significant correlation with both the degree of mononuclear cell infiltration (mostly UCHL1-positive T cells) and the total length of the carcinoma tissue in each case.
Proliferation of alphaSMA-containing stromal cells in the lamina propria may be involved in altering the endoscopic features of the esophagus in cases with intraepithelial carcinoma or an intraepithelial extension of carcinoma tissue.
消化道固有层是包含血管、肌成纤维细胞和其他间质成分的空间。本研究旨在阐明该空间内肌成纤维细胞的增殖与其他组织学特征（如炎症细胞浸润和血管增殖）之间的关系。
共38例病例，包括19例早期和19例晚期食管鳞状细胞癌伴上皮内扩展（前者包括10例原位癌病变和15例仅侵犯固有层的癌病变），采用苏木精-伊红（H&E）染色、阿赞-马洛里（Azan Mallory）染色和免疫染色，以鉴定固有层中间质细胞针对α-平滑肌肌动蛋白（αSMA）、结蛋白、波形蛋白、因子VIII、IV型胶原、层粘连蛋白或炎症细胞（L26、UCHL1、Kp1和c-kit）的特征。通过测量每例中肌成纤维细胞束的总长度来评估肌成纤维细胞的增殖潜能。
含αSMA的基质细胞增殖：i）发生在上皮内癌下方的固有层（64%）或癌组织上皮内扩展下方的固有层（47%），ii）与每例中单核细胞浸润程度（主要是UCHL1阳性T细胞）和癌组织总长度均呈显著相关。
固有层中含αSMA的基质细胞增殖可能参与改变上皮内癌或癌组织上皮内扩展病例中食管的内镜特征。
