Kuwano H, Sonoda K, Yasuda M, Sumiyoshi K, Nozoe T, Sugimachi K
Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Surg Oncol. 1997 Jul;65(3):188-93. doi: 10.1002/(sici)1096-9098(199707)65:3<188::aid-jso8>3.0.co;2-2.
Although the relationship between angiogenesis and tumor proliferation or malignant potential has been previously demonstrated in several studies, early stage of cancer invasion and angiogenesis has seldom been investigated.
From the esophageal specimens of eight recently resected cases with esophageal squamous cell carcinoma, 25 areas of carcinoma-in-situ or microinvasive carcinoma were selected, and then a serial histologic investigation and immunohistochemical staining for the detection of Factor VIII-related antigen to investigate microvessels in the lamina propria mucosae beneath the lesions as a measure of angiogeneses and staining for laminin to visualize basement membrane was performed. Lymphocyte infiltration below the lesions were also estimated. In view of early cancerous invasion, histologic patterns of the growth of the lesions were divided into "flat," "expansive," and "downgrowth" patterns.
Although downgrowth patterns are thought to be more invasive, relationships between the histologic patterns, and basement membrane staining patterns, and lymphocyte infiltration patterns were not demonstrated. However, the angiogenetic ratio (the number of vessels/cm under the lesions divided by that under normal epithelium) was observed to be significantly and closely related to tumor invasion patterns (P < 0.01), although it was not related to the destruction of the basement membrane or lymphocyte infiltration below the lesions.
The angiogenesis of esophageal squamous cell carcinoma is closely correlated to the tumor invasion patterns in early esophageal cancerous lesions.
尽管此前多项研究已证实血管生成与肿瘤增殖或恶性潜能之间的关系，但癌症侵袭与血管生成的早期阶段却鲜有研究。
从近期切除的8例食管鳞状细胞癌标本中选取25个原位癌或微浸润癌区域，随后进行系列组织学研究及免疫组化染色，检测因子VIII相关抗原以研究病变下方黏膜固有层中的微血管作为血管生成的指标，同时进行层粘连蛋白染色以观察基底膜。还对病变下方的淋巴细胞浸润情况进行评估。鉴于早期癌侵袭，将病变生长的组织学模式分为“扁平”“膨胀性”和“向下生长”模式。
尽管向下生长模式被认为侵袭性更强，但未发现组织学模式、基底膜染色模式和淋巴细胞浸润模式之间存在关联。然而，血管生成率（病变下方每厘米血管数量除以正常上皮下方的血管数量）虽与病变下方基底膜的破坏或淋巴细胞浸润无关，但与肿瘤侵袭模式显著且密切相关（P < 0.01）。
食管鳞状细胞癌的血管生成与早期食管癌病变中的肿瘤侵袭模式密切相关。
