Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin, 300193, PR China.
Phytochem Anal. 2012 Nov-Dec;23(6):668-76. doi: 10.1002/pca.2371. Epub 2012 Jun 7.
Phenylethanoid glycosides (PhGs) are the major active constituents of Cistanches deserticola Y.C. Ma. However, the isolation, purification and identification procedures of PhGs are difficult and time-consuming.
To establish a rapid and sensitive ultrahigh-pressure liquid chromatography (UHPLC)/ESI (electrospray ion source)-quadrupole time of flight (QTOF)-MS/MS method that could be applied to rapidly profile and identify PhGs.
Seven standard compounds were used for the investigation of the fragmentation pattern. Based on the UHPLC/ESI-QTOF-MS/MS method, the important structural information on the types of aglycone and saccharide sequences present should be obtained.
According to the HPLC retention behaviour, the proposed fragmentation pathways provided by high-resolution MS and MS/MS spectra and literature sources, a total of 13 PhGs in the crude extract of C. deserticola were identified or tentatively identified.
A rapid and accurate UHPLC/ESI-QTOF-MS/MS method was established for the identification of PhGs in the crude extract of C. deserticola. This method therefore can be used for rapid prediction of the chemical constituents and qualities of this plant.
苯乙醇苷类（PhGs）是肉苁蓉的主要活性成分。然而，PhGs 的分离、纯化和鉴定程序既困难又耗时。
建立一种快速灵敏的超高效液相色谱（UHPLC）/电喷雾（ESI）-四极杆飞行时间（QTOF）-MS/MS 方法，用于快速分析和鉴定 PhGs。
用七种标准化合物研究了裂解模式。基于 UHPLC/ESI-QTOF-MS/MS 方法，应该可以获得有关糖苷配基类型和糖序列存在的重要结构信息。
根据高效液相色谱保留行为、高分辨率 MS 和 MS/MS 谱以及文献来源提供的建议裂解途径，从肉苁蓉粗提取物中鉴定或初步鉴定出 13 种 PhGs。
建立了一种快速准确的 UHPLC/ESI-QTOF-MS/MS 方法，用于鉴定肉苁蓉粗提取物中的 PhGs。因此，该方法可用于快速预测该植物的化学成分和质量。
