Cui T, Zhou X, Zheng F
Department of Clinical Chemistry, Second Affiliated Hospital, Hubei Medical University, Wuhan 430071, China.
Zhonghua Yi Xue Za Zhi. 2000 Nov;80(11):838-40.
To investigate the association between the polymorphism of apolipoprotein E (apoE) intron 1 enhancer (IE1) and the level of serum lipid in patients with coronary heart disease (CHD).
ApoE IE1 genotypes of 81 patients with CHD and 108 healthy subjects were detected by polymerase chain reaction and restriction fragment length polymorphism.
The frequency of G/G genotype in patients with CHD was significantly higher than that in control group (P < 0.05). The frequency of G allele was significantly higher in patients than in control group (P < 0.05). The gene polymorphism of apoE IE1 obviously influenced the serum lipid levels. The concentrations of TC, and LDL-C among different genotype groups were significantly different, and the level in G/G group was higher than that in C/C group (P < 0.05).
ApoE IE1 gene polymorphism is closely related to serum lipid, and G/G genotype of apoE IE1 may be a risk factor of coronary heart disease in Han Chinese.
探讨载脂蛋白E（apoE）内含子1增强子（IE1）多态性与冠心病（CHD）患者血脂水平的相关性。
采用聚合酶链反应和限制性片段长度多态性方法检测81例冠心病患者和108例健康对照者的apoE IE1基因型。
冠心病患者中G/G基因型频率显著高于对照组（P<0.05）。患者中G等位基因频率显著高于对照组（P<0.05）。apoE IE1基因多态性明显影响血脂水平。不同基因型组间总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）浓度有显著差异，G/G组水平高于C/C组（P<0.05）。
apoE IE1基因多态性与血脂密切相关，apoE IE1的G/G基因型可能是汉族人群冠心病的危险因素。
