Zhang X, Liu B, Bai H, Fan P
Apolipoprotein Research Unit, West China University of Medical Sciences, Chengdu, Sichuan 610041 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001 Apr;18(2):100-4.
To investigate apolipoprotein(apo) E polymorphism and its relationship with serum lipids and apolipoproteins in Chinese patients with endogenous hypertriglyceridemia(HTG).
apoE genotype was assayed by polymerase chain reaction- restriction fragment length polymorphism(PCR-RFLP), serum lipids were determined by enzyme method, and apolipoproteins were measured by radial immunodiffusion assay in 225 endogenous HTG patients whose fasting serum TG levels were > or = 2.26 mmol/L and in 230 healthy subjects whose fasting serum lipids levels were TG<1.82 mmol/L, TC<6.21 mmol/L from a population of Chinese Han nationality in Chengdu area.
Compared with the controls, the values of BMI, TG, TC, nHDLC, apoB100, CII, CIII, E and TG/HDLC in HTG patients were significantly increased and the values of HDLC, apoAI and apoE/apoCIII were significantly decreased(P<0.001). apoE3/3 genotype and allele epsilon(3) frequency in HTG group and the control group were both the highest, and allele epsilon( 2) frequency in HTG group tended to increase than that in the control group(P>0.05). Both in HTG group and control group, the genotype of apoE2 had higher serum TG and apoE levels, lower LDLC level and decreased apoE/apoCIII ratio as compared with the genotype of apoE3 or apoE4(P<0.001).
Allele epsilon(2) of apoE gene was associated with higher serum TG and apoE levels and lower serum LDLC level, and the lower ratio of apoE/apoCIII was associated with the higher serum level of TG in endogenous HTG.
研究中国内源性高甘油三酯血症（HTG）患者载脂蛋白（apo）E基因多态性及其与血脂和载脂蛋白的关系。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测apoE基因型，用酶法测定血脂，用放射免疫扩散法测定载脂蛋白。选取成都地区汉族人群中225例空腹血清甘油三酯（TG）水平≥2.26 mmol/L的内源性HTG患者和230例空腹血脂水平TG<1.82 mmol/L、总胆固醇（TC）<6.21 mmol/L的健康对照者。
与对照组相比，HTG患者的体重指数（BMI）、TG、TC、非高密度脂蛋白胆固醇（nHDLC）、apoB100、CII、CIII、E及TG/高密度脂蛋白胆固醇（HDLC）值显著升高，HDLC、apoAI及apoE/apoCIII值显著降低（P<0.001）。HTG组和对照组中apoE3/3基因型及等位基因ε（3）频率均最高，HTG组等位基因ε（2）频率较对照组有升高趋势（P>0.05）。在HTG组和对照组中，与apoE3或apoE4基因型相比，apoE2基因型患者血清TG和apoE水平较高，低密度脂蛋白胆固醇（LDLC）水平较低，apoE/apoCIII比值降低（P<0.001）。
apoE基因的等位基因ε（2）与内源性HTG患者较高的血清TG和apoE水平及较低的血清LDLC水平相关，apoE/apoCIII比值降低与内源性HTG患者较高的血清TG水平相关。
