氢化可的松对体外衰老过程中DNA合成及细胞分裂的影响。
The effect of hydrocortisone on DNA synthesis and cell division during aging in vitro.
作者信息
Cristofalo V J
出版信息
Adv Exp Med Biol. 1975;53:7-22. doi: 10.1007/978-1-4757-0731-1_2.
Hydrocortisone (14 muM) added to cultures of human diploid fibroblast-like cells extends the lifespan of the population. This effect is expressed during a single growth cycle by an increased rate of proliferation and a higher rate of incorporation of 3HdT into DNA. The hormone appears to exert its effect by increasing the fraction of cells in the proliferating pool, and this increase is expressed at all levels of serial subcultivation (population ages). The effect requires a minimum of between 12 and 18 hr exposure of the cells to hydrocortisone. Studies with varying amounts of serum suggest that the hormone affects the cells by amplifying the serum signal which is the primary signal for cell division.
向人二倍体成纤维细胞样细胞培养物中添加氢化可的松（14微摩尔）可延长细胞群体的寿命。这种效应在单个生长周期中表现为增殖速率增加以及3HdT掺入DNA的速率更高。该激素似乎通过增加增殖池中细胞的比例来发挥其作用，并且这种增加在连续传代培养的所有水平（群体年龄）上都有体现。这种效应要求细胞至少接触氢化可的松12至18小时。用不同量血清进行的研究表明，该激素通过放大作为细胞分裂主要信号的血清信号来影响细胞。
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