Leeb I J
Am J Anat. 1975 Jan;142(1):29-45. doi: 10.1002/aja.1001420104.
The object of the present study was to establish a model for the study of parotid gland regeneration. Adult female Spague-Dalley rats were placed for 11 days on a protein-free diet with daily intraperitoneal doses of aqueous DL-ethionine equivalent to 0.5 hg/gm body weight, and returned to a normal diet on day 12. At varying intervals, both during and after intoxication, animals were sacrificed and the parotid glands prepared for study with the transmission electron microscope. Ultrastructural observations indicated that damage was essentially limited to the acinar cells, in which the protein synthetic apparatus was the focus of injury. The rough endoplasmic reticulum displayed atypical configurations, loss of attached ribosomes and membrane fragmentation. In the Golgi region, an atypical structure, a "crystalloid" arose during intoxication. Because of the morphology and apparent formation of the "crystalloid," it was assumed to be an abnormal secretion product. Resumption of a normal diet resulted in the rapid restitution of the normal cytoarchitecture. During the first week of recovery, there was prominent mitotic activity in mature acinar cells. It was concluded that the primary effect of ethionine upon the parotid gland is interference with the function of the protein-synthetic apparatus, leading to morphologic alteration of the acinar cells. The mitotic activity observed during recovery indicated that in the adult rat, acinar cells retain the potential for proliferation.
本研究的目的是建立一个用于腮腺再生研究的模型。成年雌性斯帕格 - 戴利大鼠被置于无蛋白饮食中11天，每天腹腔注射相当于0.5毫克/克体重的DL - 乙硫氨酸水溶液，在第12天恢复正常饮食。在中毒期间和中毒后不同时间间隔，处死动物并制备腮腺用于透射电子显微镜研究。超微结构观察表明，损伤主要局限于腺泡细胞，其中蛋白质合成装置是损伤的焦点。粗面内质网呈现非典型形态，附着核糖体丢失和膜断裂。在高尔基体区域，中毒期间出现一种非典型结构，即“类晶体”。由于“类晶体”的形态和明显形成过程，推测它是一种异常分泌产物。恢复正常饮食导致正常细胞结构迅速恢复。在恢复的第一周，成熟腺泡细胞中有显著的有丝分裂活性。得出的结论是，乙硫氨酸对腮腺的主要作用是干扰蛋白质合成装置的功能，导致腺泡细胞形态改变。恢复期间观察到的有丝分裂活性表明，成年大鼠的腺泡细胞保留增殖潜力。
