Kagawa Y, Hirayama K, Tagami M, Tsunoda N, Yoshino T, Matsui T, Furuoka H, Taniyama H
Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midorimachi 582-1, Ebetsu, Hokkaido 069-8501, Japan.
J Comp Pathol. 2001 Feb-Apr;124(2-3):122-7. doi: 10.1053/jcpa.2000.0439.
Histopathological and immunohistochemical examinations were made on four female horses aged 9-12 years with pulmonary granular cell tumours (GCTs). The tumours, which were multiple, of varying size, firm and off-white in colour, surrounded the bronchi and bronchioles. Metastatic lesions were not detected. The tumour cells had abundant eosinophilic cytoplasm filled with prominent coarse eosinophilic granules. Immunohistochemically, these tumour cells reacted uniformly with vimentin and S100 antibodies. Most were immunolabelled by antibodies against glial fibrillary acidic protein (GFAP), myelin basic protein (MBP) and protein gene product 9.5 (PGP9.5), and a few cells were positive with Leu7 antibody. However, the tumour cells did not react with antibodies against neurofilament protein (NF), cytokeratin (CK), chromogranin, alpha1 antichymotrypsin (AACT), myoglobin, desmin, alpha-actin or alpha-smooth muscle actin (alpha-SMA). These immunohistochemical properties of tumour cells support the hypothesis that equine pulmonary GCTs are derived from Schwann cells of the peripheral nervous system in peribronchial and peribronchiolar tissues. GFAP, MBP, Leu7 and PGP9.5 antibodies should help to distinguish equine granular cell tumours from other tumours.
对4匹年龄在9至12岁、患有肺颗粒细胞瘤（GCT）的雌性马进行了组织病理学和免疫组织化学检查。肿瘤为多发性，大小不一，质地坚硬，呈灰白色，围绕支气管和细支气管。未检测到转移病变。肿瘤细胞具有丰富的嗜酸性细胞质，充满明显的粗大嗜酸性颗粒。免疫组织化学检查显示，这些肿瘤细胞与波形蛋白和S100抗体呈一致反应。大多数肿瘤细胞被抗胶质纤维酸性蛋白（GFAP）、髓磷脂碱性蛋白（MBP）和蛋白基因产物9.5（PGP9.5）的抗体免疫标记，少数细胞对Leu7抗体呈阳性反应。然而，肿瘤细胞不与抗神经丝蛋白（NF）、细胞角蛋白（CK）、嗜铬粒蛋白、α1抗糜蛋白酶（AACT）、肌红蛋白、结蛋白、α肌动蛋白或α平滑肌肌动蛋白（α-SMA）的抗体发生反应。肿瘤细胞的这些免疫组织化学特性支持了马肺GCT起源于支气管周围和细支气管周围组织中周围神经系统雪旺细胞的假说。GFAP、MBP、Leu7和PGP9.5抗体有助于将马颗粒细胞瘤与其他肿瘤区分开来。
