Geyer C, Hafner A, Pfleghaar S, Hermanns W
Institut für Tierpathologie, Ludwig-Maximilians-Universität München.
Zentralbl Veterinarmed B. 1992 Sep;39(7):485-94. doi: 10.1111/j.1439-0450.1992.tb01197.x.
Six canine, one feline and one equine granular cell tumours (GCTs) were investigated electron microscopically and immunohistochemically. The tumours were tested for reactivity with monoclonal antibodies against vimentin and desmin and with polyclonal antibodies against cytokeratin, S-100 protein, glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE). All GCTs were characterized by their PAS positive cytoplasmic granules in light microscopy, which in electron microscopy appeared as lysosome-like granules. In each case two canine GCTs were stained by the antibody against cytokeratin, vimentin and S-100 protein. Cells of the equine GCT showed reactivity with the antiserum against S-100 protein. In the feline GCT no reactivity with any of the antibodies tested was observed. These differences of the immunohistochemical reactions of GCTs suggest a nonuniform histogenesis of GCTs in domestic animals. The reactivity of the tumour cells with the antiserum against NSE is discussed.
对6例犬、1例猫和1例马的颗粒细胞瘤（GCT）进行了电子显微镜和免疫组织化学研究。检测这些肿瘤与抗波形蛋白和结蛋白的单克隆抗体以及抗细胞角蛋白、S-100蛋白、胶质纤维酸性蛋白（GFAP）和神经元特异性烯醇化酶（NSE）的多克隆抗体的反应性。所有GCT在光学显微镜下均以其PAS阳性细胞质颗粒为特征，在电子显微镜下表现为溶酶体样颗粒。在每种情况下，2例犬GCT被抗细胞角蛋白、波形蛋白和S-100蛋白的抗体染色。马GCT的细胞与抗S-100蛋白抗血清呈反应性。在猫GCT中未观察到与任何测试抗体的反应性。GCT免疫组织化学反应的这些差异表明家畜GCT的组织发生不统一。讨论了肿瘤细胞与抗NSE抗血清的反应性。
