Haan K M, Aiyar A, Longnecker R
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
J Virol. 2001 Mar;75(6):3016-20. doi: 10.1128/JVI.75.6.3016-3020.2001.
Epstein-Barr virus (EBV) is a strict human pathogen for which no small animal models exist. Plasmids that contain the EBV plasmid origin of replication, oriP, and express EBV nuclear antigen 1 (EBNA1) are stably maintained extrachromosomally in human cells, whereas these plasmids replicate poorly in rodent cells. However, the ability of oriP and EBNA1 to maintain the entire EBV episome in proliferating rodent cells has not been determined. Expression of the two human B-cell receptors for EBV on the surfaces of murine B cells allows efficient viral entry that leads to the establishment of latent EBV infection and long-term persistence of the viral genome. Latent gene expression in these cells resembles the latency II profile in that EBNA1 and LMP1 can be detected whereas EBNA2 and the EBNA3s are not expressed.
爱泼斯坦-巴尔病毒（EBV）是一种严格的人类病原体，不存在小动物模型。含有EBV质粒复制起点oriP并表达EBV核抗原1（EBNA1）的质粒在人类细胞中能稳定地在染色体外维持，而这些质粒在啮齿动物细胞中复制较差。然而，oriP和EBNA1在增殖的啮齿动物细胞中维持整个EBV附加体的能力尚未确定。在鼠B细胞表面表达两种人类EBV B细胞受体可实现高效的病毒进入，从而导致潜伏性EBV感染的建立和病毒基因组的长期持续存在。这些细胞中的潜伏基因表达类似于潜伏期II型，即可以检测到EBNA1和LMP1，而EBNA2和EBNA3s不表达。
