微生物基因组中操纵子的预测。
Prediction of operons in microbial genomes.
作者信息
Ermolaeva M D, White O, Salzberg S L
机构信息
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
出版信息
Nucleic Acids Res. 2001 Mar 1;29(5):1216-21. doi: 10.1093/nar/29.5.1216.
Abstract
Operon structure is an important organization feature of bacterial genomes. Many sets of genes occur in the same order on multiple genomes; these conserved gene groupings represent candidate operons. This study describes a computational method to estimate the likelihood that such conserved gene sets form operons. The method was used to analyze 34 bacterial and archaeal genomes, and yielded more than 7600 pairs of genes that are highly likely (P: >/= 0.98) to belong to the same operon. The sensitivity of our method is 30-50% for the Escherichia coli genome. The predicted gene pairs are available from our World Wide Web site http://www.tigr.org/tigr-scripts/operons/operons.cgi.
摘要
操纵子结构是细菌基因组的一个重要组织特征。许多基因集在多个基因组中以相同顺序出现；这些保守的基因分组代表候选操纵子。本研究描述了一种计算方法，用于估计此类保守基因集形成操纵子的可能性。该方法被用于分析34个细菌和古细菌基因组，产生了超过7600对极有可能（P：≥0.98）属于同一操纵子的基因。我们的方法对大肠杆菌基因组的灵敏度为30%-50%。预测的基因对可从我们的万维网站点http://www.tigr.org/tigr-scripts/operons/operons.cgi获取。
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