Takaku H, Ajioka Y, Watanabe H, Hashidate H, Yamada S, Yokoyama J, Kazama S, Suda T, Hatakeyama K
First Department of Pathology, Niigata University School of Medicine, Niigata 951-8510, Japan.
Jpn J Cancer Res. 2001 Feb;92(2):119-26. doi: 10.1111/j.1349-7006.2001.tb01073.x.
Two cases of ulcerative colitis (UC)-associated carcinoma or dysplasia and morphologically non-neoplastic mucosa with p53 protein overexpression (MNNM-p53OE) were selected. DNA was extracted from the paraffin blocks of these lesions and exons 5 - 8 of the p53 gene were analyzed by PCR and direct sequencing. In addition, mutations in K-ras codon 12 were analyzed by PCR-RFLP methods. MNNM-p53OE was located surrounding and adjoining a coexisting carcinoma and / or dysplasia. A p53 mutation was detected in 12 / 22 (54.5%) MNNM-p53OE samples, 4 / 8 (50%) dysplasia samples and 8 / 8 (100%) carcinoma samples. The p53 mutations detected in MNNM-p53OE were identical to those demonstrated in the adjoining carcinoma and / or dysplasia. No K-ras codon 12 mutation was detected in any of the samples. These results indicate that MNNM-p53OE may share an identical clonal linkage with a coexisting carcinoma and / or dysplasia, and may be an initial and submorphological form of UC-associated neoplasia. Recognition of MNNM-p53OE in biopsy specimens may help to identify patients with UC at risk of developing colorectal carcinoma.
选取了两例溃疡性结肠炎（UC）相关癌或发育异常以及形态学上无肿瘤形成但p53蛋白过表达的黏膜（MNNM - p53OE）。从这些病变的石蜡块中提取DNA，通过聚合酶链反应（PCR）和直接测序分析p53基因的第5 - 8外显子。此外，通过PCR - 限制性片段长度多态性（RFLP）方法分析K - ras密码子12的突变情况。MNNM - p53OE位于共存的癌和/或发育异常周围及相邻部位。在22个MNNM - p53OE样本中有12个（54.5%）检测到p53突变，8个发育异常样本中有4个（50%）检测到p53突变，8个癌样本中有8个（100%）检测到p53突变。在MNNM - p53OE中检测到的p53突变与相邻的癌和/或发育异常中显示的突变相同。在任何样本中均未检测到K - ras密码子12突变。这些结果表明，MNNM - p53OE可能与共存的癌和/或发育异常具有相同的克隆联系，并且可能是UC相关肿瘤形成的初始和亚形态学形式。在活检标本中识别MNNM - p53OE可能有助于识别有患结直肠癌风险的UC患者。
