Kuwabara A, Watanabe H, Ajioka Y, Yasuda K, Saito H, Matsuda K, Kijima H, Hatakeyama K
First Department of Pathology, Niigata University School of Medicine.
Jpn J Cancer Res. 1998 Jan;89(1):40-6. doi: 10.1111/j.1349-7006.1998.tb00477.x.
The aim of this study was to clarify whether or not the status of gene alteration is heterogeneous in intramucosal carcinoma and homogeneous within invasive carcinoma. We selected 10 colorectal carcinoma cases (1 mucosal, 5 submucosal and 4 advanced carcinomas including 2 cases with lymph node metastasis) and analyzed the p53 gene sequence. Six colorectal cancers in this study showed heterogeneity in p53 mutations in cells from the intramucosal part. In the invasive part of a carcinoma, p53 mutation status was homogeneous intratumorally in all cases. These data indicate that, in regard to p53 gene alterations, colorectal cancers can be composed of various subclones when limited to the mucosa, but clonal selection occurs when one of these subclones commences invasion to the submucosa, generating a monoclonal invasive carcinoma.
本研究的目的是阐明基因改变状态在黏膜内癌中是否异质性，而在浸润癌内是否同质性。我们选取了10例结直肠癌病例（1例黏膜癌、5例黏膜下癌和4例进展期癌，其中2例有淋巴结转移）并分析p53基因序列。本研究中的6例结直肠癌在黏膜内部分的细胞中显示出p53突变的异质性。在癌的浸润部分，所有病例肿瘤内p53突变状态是同质性的。这些数据表明，就p53基因改变而言，当局限于黏膜时，结直肠癌可由各种亚克隆组成，但当这些亚克隆之一开始浸润至黏膜下时会发生克隆选择，从而产生单克隆浸润癌。
