Takasaki T, Takada K, Kurane I
Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
Intervirology. 2001;44(1):48-54. doi: 10.1159/000050030.
We investigated persistent infection of Epstein-Barr virus (EBV)-transformed lymphoblastoid cells with dengue virus using a transmission electron microscope. Most of the infected cells kept an intact morphology with only a few virus particles in the cytoplasm, but without any indication of active viral replication. Some cells were apoptotic and a few dengue virus particles were present in these apoptotic cells. Raji cells acutely infected with dengue-2 virus showed degenerative morphology. There were membranous tubular structures and many virus particles around and within the infected cells. Approximately 95% of the cells were dengue viral antigen positive in the persistently infected EBV-transformed cell line. It is likely that persistent infection is maintained mainly by the division of infected cells without cytopathic effect by dengue-2 virus.
我们使用透射电子显微镜研究了登革病毒对爱泼斯坦-巴尔病毒（EBV）转化的淋巴母细胞的持续感染。大多数受感染细胞保持完整形态，细胞质中只有少数病毒颗粒，但没有任何活跃病毒复制的迹象。一些细胞发生凋亡，这些凋亡细胞中存在少数登革病毒颗粒。急性感染登革2型病毒的拉吉细胞呈现退化形态。受感染细胞周围和内部有膜性管状结构和许多病毒颗粒。在持续感染的EBV转化细胞系中，约95%的细胞登革病毒抗原呈阳性。持续性感染可能主要通过受感染细胞的分裂得以维持，而不受登革2型病毒的细胞病变效应影响。
