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登革2型病毒感染培养的人单核细胞的超微结构研究

Ultrastructural studies on dengue virus type 2 infection of cultured human monocytes.

作者信息

Mosquera Jesus A, Hernandez Juan Pablo, Valero Nereida, Espina Luz Marina, Añez German J

机构信息

Seccion de Inmunologia y Biologia Celular, Instituto de Investigaciones Clinicas Dr, Americo Negrette, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela.

出版信息

Virol J. 2005 Mar 31;2:26. doi: 10.1186/1743-422X-2-26.

DOI:10.1186/1743-422X-2-26
PMID:15801983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1082913/
Abstract

BACKGROUND

Early interaction of dengue virus and monocyte/macrophages could be an important feature for virus dissemination after its initial entry via the mosquito vector. Since ultrastructural analysis of this interaction has not been reported, dengue type 2 (DEN2) virus-infected human monocyte cultures were studied at 1, 2, 4 and 6 hours after infection.

RESULTS

Typical dengue particles and fuzzy coated viral particles were 35 to 42 nm and 74 to 85 nm respectively. Viruses were engulfed by phagocytosis and macropicnocytosis leading to huge vacuoles and phagosomes inside the monocytes. Interaction of monocytes with DEN2 virus induced apoptosis, characterized by nuclear condensation and fragmentation, cellular shrinkage, blebbing and budding phenomena and phagocytosis of apoptotic cells by neighboring monocytes. This finding was confirmed by TUNEL. Ultrastructural features associated to DEN2 virus replication were not observed.

CONCLUSION

These data suggest that clearance of the virus by monocytes and cellular death are the main features during the initial interaction of DEN2 virus and monocytes and this could be important in the rapid elimination of the virus after infection by mosquito vector.

摘要

背景

登革病毒与单核细胞/巨噬细胞的早期相互作用可能是病毒通过蚊媒初次进入人体后进行传播的一个重要特征。由于尚未见有关这种相互作用的超微结构分析报道，因此我们对登革2型（DEN2）病毒感染的人单核细胞培养物在感染后1、2、4和6小时进行了研究。

结果

典型的登革病毒颗粒和模糊包膜病毒颗粒分别为35至42纳米和74至85纳米。病毒通过吞噬作用和巨吞饮作用被单核细胞吞噬，导致单核细胞内出现巨大的液泡和吞噬体。单核细胞与DEN2病毒的相互作用诱导细胞凋亡，其特征为核浓缩和碎片化、细胞收缩、出泡和芽生现象以及邻近单核细胞对凋亡细胞的吞噬。这一发现通过TUNEL得到证实。未观察到与DEN2病毒复制相关的超微结构特征。

结论

这些数据表明，单核细胞清除病毒和细胞死亡是DEN2病毒与单核细胞初次相互作用期间的主要特征，这可能对蚊媒感染后迅速清除病毒具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/92342095c539/1743-422X-2-26-10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/91e6d879264d/1743-422X-2-26-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/4f42a6396c42/1743-422X-2-26-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/75c2f5669e0c/1743-422X-2-26-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/3449c2d5739e/1743-422X-2-26-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/73a496d9d532/1743-422X-2-26-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/aa257cce2051/1743-422X-2-26-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/eadf5f3854b0/1743-422X-2-26-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/267b81e81c4d/1743-422X-2-26-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/d6529f0d0451/1743-422X-2-26-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/92342095c539/1743-422X-2-26-10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/91e6d879264d/1743-422X-2-26-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/4f42a6396c42/1743-422X-2-26-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/75c2f5669e0c/1743-422X-2-26-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/3449c2d5739e/1743-422X-2-26-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/73a496d9d532/1743-422X-2-26-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/aa257cce2051/1743-422X-2-26-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/eadf5f3854b0/1743-422X-2-26-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/267b81e81c4d/1743-422X-2-26-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/d6529f0d0451/1743-422X-2-26-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b947/1082913/92342095c539/1743-422X-2-26-10.jpg

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