Sarica K, Bakir K, Erbagci A, Yagci F, Uysal O, Uçak R
Department of Urology, Sahinbey Hospital, University of Gaziantep, Medical School, TR-27070 Kolejtepe/Gaziantep, Turkey.
Urol Int. 2001;66(2):94-9. doi: 10.1159/000056578.
The present study aims to evaluate the histologic as well as apoptotic changes indicated by PCNA, p-53 and bcl-2 expression in the contralateral testes after a period of unilateral testicular torsion of 4 h.
Regarding the histologic changes, while some significant alterations such as complete or incomplete spermatocytic arrest as well as sertoli cell only formation have to some extent been noted after the detorsion procedure, reasonably better preserved histology could be demonstrated following the orchiectomy procedure both in the early and late follow-up. Thus, the orchiectomy procedure was found to be limiting enough on the severity of the histologic changes in the contralateral testes after a certain period of time following the torsion procedure. On the other hand, however, in relation to the apoptotic events indicated by the three markers, while PCNA activity was found to be significantly different depending on the procedure performed (detorsion or orchiectomy), p-53 and bcl-2 positivity did not exhibit any difference in this aspect. Increased PCNA activity (especially after the detorsion procedure) together with marked positivity of p-53 both in the early and late follow-up indicated the increased cell turnover in the contralateral testes, which in turn may be accepted as a sign of increased apoptosis in these testes. In addition to these findings, bcl-2 expression has been found to be consistently negative in all specimens evaluated both in the early and late follow-up.
Taking into account the strong inhibitory effect of bcl-2 during apoptotic events, these findings again support the likelihood of increased apoptosis in the contralateral testes.
本研究旨在评估单侧睾丸扭转4小时后对侧睾丸中由PCNA、p-53和bcl-2表达所指示的组织学及凋亡变化。
关于组织学变化，虽然在扭转解除手术后在一定程度上已观察到一些显著改变，如完全或不完全的生精细胞停滞以及仅支持细胞形成，但在早期和晚期随访中，睾丸切除术后的组织学保存情况相对较好。因此，发现在扭转手术后一段时间后，睾丸切除术对侧睾丸组织学变化的严重程度具有足够的限制作用。然而，另一方面，就这三种标志物所指示的凋亡事件而言，虽然发现PCNA活性根据所执行的手术（扭转解除或睾丸切除术）存在显著差异，但p-53和bcl-2的阳性在这方面未表现出任何差异。PCNA活性增加（尤其是在扭转解除手术后）以及在早期和晚期随访中p-53的明显阳性表明对侧睾丸中的细胞更新增加，这反过来可能被视为这些睾丸中凋亡增加的一个迹象。除这些发现外，在早期和晚期随访中评估的所有标本中，bcl-2表达一直为阴性。
考虑到bcl-2在凋亡事件中的强大抑制作用，这些发现再次支持对侧睾丸凋亡增加的可能性。
