Bray A E, Holt P G, Roberts L M, Keast D
Int J Cancer. 1975 Oct 15;16(4):607-15. doi: 10.1002/ijc.2910160410.
The early onset of serum blocking of host cell-mediated immunity was investigated in a transplantable murine melanoma employing two in vitro assays. One of these was based on the Takasugi and Klein cytotoxicity test; the other was a new test based on the inhibition of adherence of leukocytes to a plastic surface in the presence of tumor antigen. Both tests yielded similar results, and the adherence inhibition test was more sensitive. Blocking could be detected as early as 1 h after the inoculation of 1 times 10(7) melanoma cells. Early onset of serum blocking was also demonstrable if homogenized of tissue-cultured tumor cells were inoculated. Serum blocking under these circumstances was initially transient, declining at 24 to 48 h, but reappearing by the 4th day after inoculation. Furthermore, a functional immune system was not required for blocking factor development in this system, as evidenced by the onset of a similar pattern of blocking activity in heavily irradiated animals.
利用两种体外试验，对可移植性小鼠黑色素瘤中宿主细胞介导免疫的血清阻断早期发作进行了研究。其中一种试验基于高杉和克莱因细胞毒性试验；另一种是基于在肿瘤抗原存在下白细胞对塑料表面黏附抑制的新试验。两种试验结果相似，且黏附抑制试验更敏感。在接种1×10⁷个黑色素瘤细胞后1小时即可检测到阻断现象。如果接种组织培养肿瘤细胞的匀浆，也可证明血清阻断的早期发作。在这些情况下，血清阻断最初是短暂的，在24至48小时下降，但在接种后第4天再次出现。此外，该系统中阻断因子的产生不需要功能性免疫系统，重度照射动物中出现类似的阻断活性模式即可证明。
