Ionac M, Laskay T, Labahn D, Geisslinger G, Solbach W
Institute for Experimental and Clinical Pharmacology and Toxicology, University of Erlangen-Nürnberg, Germany.
J Immunol Methods. 1997 Mar 10;202(1):35-40. doi: 10.1016/s0022-1759(96)00226-8.
Several experimental approaches have been used in the past for the cannulation of the thoracic duct in mice. Most, however, are characterized by a modest reproducibility and a low lymph yield. Here, we describe a cannulation technique modified with respect to the anesthesia, the use of a silicone cannula and a simple and efficient intraabdominal fixation of the drain. Surgery averaged 45 min with an intra- and postoperative mortality rate of zero. Postoperatively, mice were given access to an exercise wheel allowing increased mobility and consequently a good lymph flow, thus maintaining the function of the cannula. The mice yielded a mean of 29.3 ml/24 h (range 8-40 ml) thoracic duct lymph, which contained a mean of 2.2 x 10(6) lymphocytes/ml during the first 24 h, decreasing to 0.1 x 10(6) lymphocytes/ml on the 2nd day after cannulation. Patency of the cannulae was 100% after 3 days. Interestingly, we have detected strain dependent differences in the anatomy of the thoracic duct in the mouse and these need to be considered when cannulation procedures are attempted.
过去已采用多种实验方法对小鼠胸导管进行插管。然而，大多数方法的特点是可重复性一般且淋巴产量较低。在此，我们描述一种插管技术，该技术在麻醉、硅胶插管的使用以及引流管简单有效的腹腔内固定方面有所改进。手术平均时长为45分钟，术中和术后死亡率均为零。术后，让小鼠使用运动轮，这能增加其活动能力，从而实现良好的淋巴液流动，进而维持插管功能。小鼠胸导管淋巴液平均产量为29.3毫升/24小时（范围为8 - 40毫升），在插管后的头24小时内，每毫升淋巴液平均含有2.2×10⁶个淋巴细胞，插管后第二天降至0.1×10⁶个淋巴细胞/毫升。插管3天后，插管通畅率为100%。有趣的是，我们检测到小鼠胸导管解剖结构存在品系依赖性差异，在尝试插管操作时需要考虑这些差异。
