Drenckhahn D, Gröschel-Stewart U
Cell Tissue Res. 1977 Jul 19;181(4):493-503. doi: 10.1007/BF00221771.
Myosin and actin were localized by indirect immunofluorescence microscopy using specific antibodies prepared in rabbits against highly purified gizzard myosin and actin. A strong fluorescence staining with both antibodies was observed in rat corneal epithelial cells, anterior lens epithelial cells, rod inner segments, and in rat and frog pigment epithelial cells. The immunohistochemical localization of myosin in corneal epithelial cells was further supported by the electrophoretic and immunological identification of smooth muscle type myosin heavy chain in pure corneal epithelial abrasions. Electron-microscopic observations revealed a clear correlation between staining with actin antibodies and the presence of numerous thin cytoplasmic filaments (50-80 A in diameter). The functional and biochemical nature of 90-110 A filaments occurring in corneal and lens epithelial cells, as well as the ultrastructural localization of myosin in ocular nonmuscle cells under study remains obscure.
使用针对高度纯化的鸡胗肌球蛋白和肌动蛋白制备的兔特异性抗体，通过间接免疫荧光显微镜对肌球蛋白和肌动蛋白进行定位。在大鼠角膜上皮细胞、晶状体前上皮细胞、视杆细胞内节以及大鼠和青蛙色素上皮细胞中均观察到两种抗体的强荧光染色。纯角膜上皮擦伤中平滑肌型肌球蛋白重链的电泳和免疫鉴定进一步支持了肌球蛋白在角膜上皮细胞中的免疫组织化学定位。电子显微镜观察显示，肌动蛋白抗体染色与大量细细胞质丝（直径50 - 80埃）的存在之间存在明显相关性。角膜和晶状体上皮细胞中出现的90 - 110埃细丝的功能和生化性质，以及所研究的眼非肌肉细胞中肌球蛋白的超微结构定位仍不清楚。
