Reynolds M T, Falkiner F R, Hardy R, Keane C T
J Med Microbiol. 1979 Aug;12(3):379-82. doi: 10.1099/00222615-12-3-379.
Eighty-six clinical isolates of fluorescent pseudomonads that did not produce pyocyanin on Diagnostic Sensitivity Test Agar or Cetrimide Agar were identified on the basis of their antibiotic sensitivity, production of pigment on King's "A" medium, growth at 42 degrees C, production of lecithinase and hydrolysis of gelatin. The identity of the strains was confirmed in tests with the ammonium salt sugars ethanol, glucose and mannitol. These tests were adequate for distinguishing between the three important fluorescent pseudomonads. The detection of casein hydrolysis on milk agar was assessed as a rapid method of distinguishing P. aeruginosa from the other species of fluorescent pseudomonads but proved unhelpful when compared with, or included in, a small set of tests. Most strains of P. aeruginosa and P. fluorescens hydrolysed casein.
在诊断敏感性试验琼脂或溴化十六烷基三甲铵琼脂上不产生绿脓菌素的86株荧光假单胞菌临床分离株，根据其抗生素敏感性、在King氏“A”培养基上产生色素、在42℃生长、产生卵磷脂酶和明胶水解情况进行鉴定。通过对铵盐糖乙醇、葡萄糖和甘露醇的试验确认了菌株的身份。这些试验足以区分三种重要的荧光假单胞菌。在牛奶琼脂上酪蛋白水解的检测被评估为一种将铜绿假单胞菌与其他荧光假单胞菌区分开来的快速方法，但与一小套试验相比或纳入其中时，证明并无帮助。大多数铜绿假单胞菌和荧光假单胞菌菌株可水解酪蛋白。
