Brown M R, Foster J H
J Clin Pathol. 1970 Mar;23(2):172-7. doi: 10.1136/jcp.23.2.172.
An agar medium containing 10% defatted milk has been tested as a diagnostic medium for Pseudomonas aeruginosa and, in particular, for differentiating between P. aeruginosa and P. fluorescens. Only P. aeruginosa colonies gave clear zones due to hydrolysis of casein together with diffused green pigment. Results on milk agar correlated well with the pattern of results from a variety of conventional tests used to identify this organism. Pigment production of P. aeruginosa on milk agar was better than on special media commonly used to enhance this characteristic. Routine diagnosis of P. aeruginosa is recommended by streaking on a solid medium containing 10% defatted milk granules, 25% nutrient broth, and 2% agar, and examining for clear zones and pigment after 24 hours' incubation.
一种含有10%脱脂乳的琼脂培养基已作为铜绿假单胞菌的诊断培养基进行了测试，特别是用于区分铜绿假单胞菌和荧光假单胞菌。由于酪蛋白水解以及扩散的绿色色素，只有铜绿假单胞菌菌落产生了清晰的区域。在乳琼脂上的结果与用于鉴定该菌的各种常规测试的结果模式相关性良好。铜绿假单胞菌在乳琼脂上的色素产生情况优于常用于增强此特征的特殊培养基。建议通过在含有10%脱脂乳颗粒、25%营养肉汤和2%琼脂的固体培养基上划线，并在培养24小时后检查是否有清晰区域和色素，来对铜绿假单胞菌进行常规诊断。
