Patsoula E, Loutradis D, Drakakis P, Kallianidis K, Bletsa R, Michalas S
First Department of Obstetrics and Gynaecology, Athens University Medical School, IVF Unit, "Alexandra" Maternity Hospital, Athens, Greece.
Reproduction. 2001 Mar;121(3):455-61. doi: 10.1530/rep.0.1210455.
The gonadotrophins LH and FSH are known to regulate gonadal growth, and differentiation, endocrine function and gametogenesis. The LH receptor is expressed in ovarian theca, granulosa and luteal cells, and in testicular Leydig cells. The FSH receptor is expressed only in ovarian granulosa cells and in testicular Sertoli cells. The expression of the FSH and LH receptors was analysed by RT-PCR to study the role of these receptors in early mouse development. After reverse transcription, strategically designed nested primers were used for amplification from cDNA. Transcripts for the receptors were present in mouse oocytes and preimplantation embryos. The presence of mRNA for FSH and LH receptors in oocytes, zygotes and preimplantation embryos indicates a potential role for the gonadotrophins in the modulation of meiotic resumption and completion of oocyte maturation, as well as a beneficial effect on early embryonic development in mice.
已知促性腺激素LH和FSH可调节性腺的生长、分化、内分泌功能及配子发生。LH受体表达于卵巢的卵泡膜细胞、颗粒细胞和黄体细胞以及睾丸的间质细胞。FSH受体仅表达于卵巢颗粒细胞和睾丸支持细胞。通过逆转录聚合酶链反应（RT-PCR）分析FSH和LH受体的表达，以研究这些受体在小鼠早期发育中的作用。逆转录后，使用精心设计的巢式引物从cDNA进行扩增。受体的转录本存在于小鼠卵母细胞和植入前胚胎中。卵母细胞、受精卵和植入前胚胎中存在FSH和LH受体的mRNA，这表明促性腺激素在调节减数分裂恢复和卵母细胞成熟完成方面具有潜在作用，并且对小鼠早期胚胎发育具有有益影响。
