Čikoš Š, Czikková S, Chrenek P, Makarevich A V, Burkuš J, Janštová Ž, Fabian D, Koppel J
Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovakia.
Reprod Domest Anim. 2014 Feb;49(1):92-100. doi: 10.1111/rda.12233. Epub 2013 Aug 31.
Catecholamines play an important role in embryogenesis, and data obtained in the rodent model indicate that they can act even during the preimplantation period of development. Using RT-PCR with specific oligonucleotide primers distinguishing among all members of the adrenergic receptor family, we examined expression of adrenergic receptors in bovine and rabbit oocytes, morulas and blastocysts. We found several profiles of adrenoceptor mRNA expression. Transcripts for some receptor subtypes (bovine alpha 2 receptors, rabbit α2A, α2C, β1 and β2 receptors) were detected at all examined stages, which suggests receptor expression throughout (or at most stages) the preimplantation developmental period. Expression in oocytes but not at later stages was found in only one adrenoceptor subtype (rabbit α1B). In contrast, mRNA for several adrenoceptors was found in embryos but not in oocytes (bovine beta adrenoceptors and rabbit α1A). Nucleotide sequences of our PCR products amplified in rabbit oocytes, and preimplantation embryos represent the first published mRNA sequences (partial sequences coding at least one transmembrane region) of rabbit α2C, β1 and β2 adrenoceptors. Our results suggest that the expression of adrenergic receptors can be a general feature of mammalian oocytes and preimplantation embryos. On the other hand, comparison of three mammalian species (cattle, rabbit and mouse) revealed possible interspecies differences in the expression of particular adrenoceptor subtypes. Our results support the opinion that stress mediators can act directly in cells of preimplantation embryos.
儿茶酚胺在胚胎发育过程中发挥着重要作用，在啮齿动物模型中获得的数据表明，它们甚至在发育的植入前期就能发挥作用。我们使用能区分肾上腺素能受体家族所有成员的特异性寡核苷酸引物进行逆转录聚合酶链反应（RT-PCR），检测了牛和兔的卵母细胞、桑椹胚和囊胚中肾上腺素能受体的表达。我们发现了几种肾上腺素能受体mRNA的表达模式。在所有检测阶段都检测到了某些受体亚型的转录本（牛α2受体、兔α2A、α2C、β1和β2受体），这表明在植入前发育阶段的整个时期（或大多数阶段）都有受体表达。仅在一种肾上腺素能受体亚型（兔α1B）中发现其在卵母细胞中有表达，但在后期阶段没有。相反，在胚胎中发现了几种肾上腺素能受体的mRNA，但在卵母细胞中未发现（牛β肾上腺素能受体和兔α1A）。我们在兔卵母细胞和植入前胚胎中扩增的PCR产物的核苷酸序列代表了兔α2C、β1和β2肾上腺素能受体首次发表的mRNA序列（编码至少一个跨膜区域的部分序列）。我们的结果表明，肾上腺素能受体的表达可能是哺乳动物卵母细胞和植入前胚胎的一个普遍特征。另一方面，对三种哺乳动物（牛、兔和小鼠）进行比较发现，特定肾上腺素能受体亚型的表达可能存在种间差异。我们的结果支持应激介质可直接作用于植入前胚胎细胞的观点。
